This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research article
Mengfei Li
Gansu Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3035-4427
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The root of the perennial herb Angelica sinensis is a widely used source for traditional Chinese medicines. While the plant thrives in cool-moist regions of western China, early bolting and flowering (EBF) for young plants, significantly reduces root quality and yield. Approaches to inhibit EBF by changes in physiology during the vernalization process have been investigated, however the mechanism for activating EBF has not been identified. Here, transcript profiles for bolted and unbolted plants (BP and UBP, respectively) are compared.
Results: A total of over 72,000 unigenes were detected with ca. 2,600 differentially expressed genes (DEGs) observed in the BP compared with UBP. While various signaling pathways participate in flower induction, it is genes associated with floral development and the sucrose pathway that are observed to be coordinated in EBF plants, to coherently up and down regulate flowering genes that activate and inhibit flowering, respectively. Down-stream signal accumulation including gibberellic acids and sucrose metabolites were also monitored by HPLC-MS/MS for EBF plants.
Conclusions: The signature transcripts pattern for the developmental pathways that drive flowering provides insight into the molecular signals that activate plant EBF.
Keywords
Angelica sinensis, early bolting and flowering, transcriptomic analysis, gibberellin metabolism, sucrose metabolism
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryMaterials.docx
Fig. S1 Growth and development of A. sinensis in a commercial production process. Fig. S2 Length distribution of assembled unigenes. Fig. S3 Read distribution of unigenes Fig. S4 Basic annotation for all unigenes. Fig. S5 Top 10 plant species distribution of the total homologous sequences. Fig. S6 Distribution of unigenes in the transcriptome with KOG functional classification. Sequences have a KOG classification among 25 categories. Fig. S7 Functional classifications of GO terms of all assembled unigenes. Fig. S8 Number of DEGs, correlation and volcano plot for unbolted versus bolted plants. Fig. S9 The GA biosynthesis pathways and the differentially expressed genes are highlighted in red. Fig. S10 Two-year-old bolted and unbolted plants of A. sinensis. Bar represents 5 cm. Fig. S11 The cluster analysis of the 40 DEGs comparing with the families in Arabidopsis thaliana. Fig. S12 Representative chromatograms of reference standard of GA1, GA4, GA8, GA9 and GA20. Fig. S13 Representative chromatograms of bolted (A) and unbolted plants (B) of A. sinensis. Table S1. Calibration curves of GA1, GA4, GA8, GA9 and GA20
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Mengfei Li
Gansu Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3035-4427
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The root of the perennial herb Angelica sinensis is a widely used source for traditional Chinese medicines. While the plant thrives in cool-moist regions of western China, early bolting and flowering (EBF) for young plants, significantly reduces root quality and yield. Approaches to inhibit EBF by changes in physiology during the vernalization process have been investigated, however the mechanism for activating EBF has not been identified. Here, transcript profiles for bolted and unbolted plants (BP and UBP, respectively) are compared.
Results: A total of over 72,000 unigenes were detected with ca. 2,600 differentially expressed genes (DEGs) observed in the BP compared with UBP. While various signaling pathways participate in flower induction, it is genes associated with floral development and the sucrose pathway that are observed to be coordinated in EBF plants, to coherently up and down regulate flowering genes that activate and inhibit flowering, respectively. Down-stream signal accumulation including gibberellic acids and sucrose metabolites were also monitored by HPLC-MS/MS for EBF plants.
Conclusions: The signature transcripts pattern for the developmental pathways that drive flowering provides insight into the molecular signals that activate plant EBF.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryMaterials.docx
Fig. S1 Growth and development of A. sinensis in a commercial production process. Fig. S2 Length distribution of assembled unigenes. Fig. S3 Read distribution of unigenes Fig. S4 Basic annotation for all unigenes. Fig. S5 Top 10 plant species distribution of the total homologous sequences. Fig. S6 Distribution of unigenes in the transcriptome with KOG functional classification. Sequences have a KOG classification among 25 categories. Fig. S7 Functional classifications of GO terms of all assembled unigenes. Fig. S8 Number of DEGs, correlation and volcano plot for unbolted versus bolted plants. Fig. S9 The GA biosynthesis pathways and the differentially expressed genes are highlighted in red. Fig. S10 Two-year-old bolted and unbolted plants of A. sinensis. Bar represents 5 cm. Fig. S11 The cluster analysis of the 40 DEGs comparing with the families in Arabidopsis thaliana. Fig. S12 Representative chromatograms of reference standard of GA1, GA4, GA8, GA9 and GA20. Fig. S13 Representative chromatograms of bolted (A) and unbolted plants (B) of A. sinensis. Table S1. Calibration curves of GA1, GA4, GA8, GA9 and GA20
Loading...