Acknowledgements: We are grateful to all reviewers for their valuable comments on the manuscript.
Author Contributions: KZ and XL conceived and designed the work. HQ, ZZ, and JY collected the samples. KZ performed the experiments and analyzed the data; KZ and LL wrote the manuscript; ZL and XL revised the manuscript. All authors gave final approval of the paper.
Funding: This work was supported by the Tibet Autonomous Region Office and School Joint Foundation (Grant No. XZ202001ZR0038G), The National Natural Science Foundation of China (Grant No. 81660628) , The Special Support Plan for the Talent of Fifth WANREN Plan in China（Grant No.2020-366）,The Forth National Survey of Traditional Chinese Medicine Resources, Chinese or Tibet Medicinal Resources Investigation in Tibet Autonomous Region (State Administration of Chinese Traditional Medicine 20190411-542222), The Key R & D program of Yunnan Province, China (grant no. 202103AC100003), The Large-scale Scientific Facilities of the Chinese Academy of Sciences (Grant No. 2017-LSF-GBOWS-02).
Availability of data and materials: The datasets supporting the results of this publication are included within the article and Additional files1,2,3,4,5. The chloroplast genome data of 6 cp genome sequences of Rhodiola used for analysis could be obtained from NCBI, and their accession numbers are as follow: R. wallichiana, OL742458; R. henryi, OL742459; R. gelida, OL742460; R. bupleuroides, OL742461; R. tangutica, OL742462; R. quadrifida, OL742463. Data for our newly assembled six Rhodiola chloroplast genomes are also available in Supplementary S6. Voucher specimens of 6 Rhodiola were deposited in the Kunming Institute of Botany, and their accession numbers are as follow: R. wallichiana, WH-2013-029; R. henryi, GanQL691; R. gelida, ChenSL1377; R. bupleuroides, LiuJQ-08XZ-062; R. tangutica, ChenSL0277; R. quadrifida, ChenSL0149.
Ethics approval and consent to participate
Not applicable. No specific permits were required for the collection of specimens for this study. This research was carried out in compliance with the relevant laws of China.
Consent for publication
The authors declare that they have no competing interest.
1The Provincial and Ministerial co-founded collaborative innovation center for R & D in Tibet characteristic Agricultural and Animal Husbandry resources, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China
2Key Laboratory of Forest Ecology in Tibet Plateau, Tibet Agricultural & Animal Husbandry University, Ministry of Education, Nyingchi, Tibet 860000, China
3The Center for Xizang Chinese (Tibetan) Medicine Resource, Tibet Agriculture and Animal Husbandry University, Nyingchi of Tibet 860000, China
4Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China
5 The Provincial and Ministerial co-founded collaborative innovation center for R & D in Tibet characteristic Agricultural and Animal Husbandry resources, School of Life Sciences, Southwest University, Chongqing 400715, China