This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Gabriela Rosas-Salgado
Universidad Autónoma del Estado de Morelos
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Endothelial dysfunction (ED) is a key factor for the development of cardiovascular diseases. Due to its chronic, life-threatening nature, ED only can be studied experimentally in animal models. Therefore, this work was aimed to characterize a murine model of ED induced by a daily intraperitoneal administration of angiotensin II (AGII) for 10 weeks. Oxidative stress, inflammation, vascular remodeling, hypertension, and damage to various target organs were evaluated in treated animals. The results indicated that a chronic intraperitoneal administration of AGII increases the production of ROS, ICAM-1 expression, the production of TNFα, IL1β, IL17A, IL4, TGFβ, and IL10, as well as blood pressure levels; it also promotes vascular remodeling and induces non-alcoholic fatty liver disease, glomerulosclerosis, and proliferative retinopathy. Therefore, the model herein proposed can be a representative model for ED; additionally, it is easy to implement, safe, rapid, and inexpensive.
Keywords
Endothelial dysfunction, cardiovascular, SAHT
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 04 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 15 Jan, 2021
Reviews received
Received 04 Jan, 2021
Reviewers agreed
On 04 Jan, 2021
Reviews received
Received 06 Dec, 2020
Reviewers agreed
On 02 Dec, 2020
Reviewers agreed
On 26 Nov, 2020
Reviewers invited
Invitations sent on 26 Nov, 2020
Editor assigned
On 26 Nov, 2020
Editor invited
On 26 Nov, 2020
Submission checks complete
On 26 Nov, 2020
First submitted
On 24 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Cell Biology & Physiology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Gabriela Rosas-Salgado
Universidad Autónoma del Estado de Morelos
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 04 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 15 Jan, 2021
Reviews received
Received 04 Jan, 2021
Reviewers agreed
On 04 Jan, 2021
Reviews received
Received 06 Dec, 2020
Reviewers agreed
On 02 Dec, 2020
Reviewers agreed
On 26 Nov, 2020
Reviewers invited
Invitations sent on 26 Nov, 2020
Editor assigned
On 26 Nov, 2020
Editor invited
On 26 Nov, 2020
Submission checks complete
On 26 Nov, 2020
First submitted
On 24 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Cell Biology & Physiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Endothelial dysfunction (ED) is a key factor for the development of cardiovascular diseases. Due to its chronic, life-threatening nature, ED only can be studied experimentally in animal models. Therefore, this work was aimed to characterize a murine model of ED induced by a daily intraperitoneal administration of angiotensin II (AGII) for 10 weeks. Oxidative stress, inflammation, vascular remodeling, hypertension, and damage to various target organs were evaluated in treated animals. The results indicated that a chronic intraperitoneal administration of AGII increases the production of ROS, ICAM-1 expression, the production of TNFα, IL1β, IL17A, IL4, TGFβ, and IL10, as well as blood pressure levels; it also promotes vascular remodeling and induces non-alcoholic fatty liver disease, glomerulosclerosis, and proliferative retinopathy. Therefore, the model herein proposed can be a representative model for ED; additionally, it is easy to implement, safe, rapid, and inexpensive.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
Loading...