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Research article
Jeong Hyun Kang
Ajou University School of Medicine
Corresponding Author
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Background
Early onset of the disease and female preponderance are the unique features of the temporomandibular joint (TMJ) osteoarthritis (OA). The immune modulation mechanisms related to etiology of OA from other joints such as knee or hip have been suggested, but the immune-associated pathophysiology of TMJ OA, especially in young females, has not been elucidated. The present study aimed to investigate the immune-related pathophysiology of TMJ OA by analyzing transcriptional profiles of peripheral blood mononuclear cells which identify the differentially expressed genes (DEGs) in young females with TMJ OA.
Methods
RNA-sequencing (RNA-seq) was conducted on 24 young females with TMJ OA (mean age 19.3 ± 3.1 years) (RNAOA) and 11 age and sex matched healthy control (mean age 20.5 ± 3.7 years) (CON). RNA-seq datasets were analyzed to identify genes, pathways, and regulatory networks of those who were involved in the development of TMJ OA.
Results
RNA-seq data analysis revealed 41 DEGs between RNAOA and CON. A total of 16 gene ontology (GO) terms including 3 molecular and 13 biological terms were annotated via the GO function of molecular function and biological process. Through ingenuity pathway analysis (IPA), 21 annotated categories of diseases and functions were identified. There were six hub genes which showed significant results in both GO enrichment analysis and IPA, namely HLA-C, HLA-F, CXCL8, IL11RA, IL13RA1, and FCGR3B.
Conclusions
The young females with TMJ OA showed alterations of the gene related to immune function in the blood and some of the changes may reflect inflammation, auto-reactivity, and altered T cell functions.
Keywords
temporomandibular joint, osteoarthritis, transcriptome, blood
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Jeong Hyun Kang
Ajou University School of Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Early onset of the disease and female preponderance are the unique features of the temporomandibular joint (TMJ) osteoarthritis (OA). The immune modulation mechanisms related to etiology of OA from other joints such as knee or hip have been suggested, but the immune-associated pathophysiology of TMJ OA, especially in young females, has not been elucidated. The present study aimed to investigate the immune-related pathophysiology of TMJ OA by analyzing transcriptional profiles of peripheral blood mononuclear cells which identify the differentially expressed genes (DEGs) in young females with TMJ OA.
Methods
RNA-sequencing (RNA-seq) was conducted on 24 young females with TMJ OA (mean age 19.3 ± 3.1 years) (RNAOA) and 11 age and sex matched healthy control (mean age 20.5 ± 3.7 years) (CON). RNA-seq datasets were analyzed to identify genes, pathways, and regulatory networks of those who were involved in the development of TMJ OA.
Results
RNA-seq data analysis revealed 41 DEGs between RNAOA and CON. A total of 16 gene ontology (GO) terms including 3 molecular and 13 biological terms were annotated via the GO function of molecular function and biological process. Through ingenuity pathway analysis (IPA), 21 annotated categories of diseases and functions were identified. There were six hub genes which showed significant results in both GO enrichment analysis and IPA, namely HLA-C, HLA-F, CXCL8, IL11RA, IL13RA1, and FCGR3B.
Conclusions
The young females with TMJ OA showed alterations of the gene related to immune function in the blood and some of the changes may reflect inflammation, auto-reactivity, and altered T cell functions.
Figure 1
Figure 2
Figure 3
Figure 4
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