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Research Article
Yuanyuan Dong
Jilin Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Oil crop varieties are currently subjected to an increasing worldwile demand and the tiger nut (Cyperus esculentus L.) attracts significant attention because of its huge capacity of lipids production. In eukaryotic cells, the balance between the accumulation of lipids and the distribution of certain pivotal molecules is fundamental for the regulation of many complex transcriptional regulatory networks. However, many studies have struggled to understand the dynamic of lipids and the transcriptomic mechanisms governing their biosynthesis and accumulation process during plant development.
Results: Our results displayed dynamic patterns for key lipids like glycolipid, phospholipid, and glyceride during the development of tiger nut tubers. Lipidomic analysis showed molecular species distribution of lipid class during developing stages. Here, we also characterrized transcription profiles of key transcripts that determined biosynthesis and distribution of natural lipids in tiger nuts tuber. The expression of FAD2 exhibited a significant influence on the molecular composition of phosphatidylcholines (PC) and phosphatidyl ethanolamine (PE) in tiger nuts. Moreover, during lipids accumulation, the expression pattern of three candidate transcripts of oleosin genes (OLE9, OLE10 and OLE11) also displayed significant leverage on the size of lipid drops.
Conclusion: We described the significant alterations in the composition of lipids class during developing stages of tiger nut tuber, we also revealed transcriptional profiles of genes invloed in lipid biosynthesis and accumulation. These results provided new landscapes for research on lipid composition, synthesis and accumulation during different developmental stages of plant tubers.
Keywords
lipidomics, lipid accumulation, Cyperus esculentus, triacylglycerol, Glycerophospholipid, RNA-seq, lipid drops
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This is a list of supplementary files associated with this preprint. Click to download.
- FigureS1.jpg
Supplementary figure 1: Percentage of all molecular species of TAG in different developing stages.
- FigureS1.jpg
Supplementary figure 1: Percentage of all molecular species of TAG in different developing stages.
- FigureS2.jpg
Supplementary figure 2: Percentage of total molecular species of DG during five developing stages of tuber.
- FigureS2.jpg
Supplementary figure 2: Percentage of total molecular species of DG during five developing stages of tuber.
- FigureS3.jpg
Supplementary figure 3: Heatmap of all molecular species compotent of PE in five developing stages of tuber.
- FigureS3.jpg
Supplementary figure 3: Heatmap of all molecular species compotent of PE in five developing stages of tuber.
- FIgureS4.jpg
Supplementary figure 4: Compotent of total molecular species profiles of PC during different developing stages of tuber.
- FIgureS4.jpg
Supplementary figure 4: Compotent of total molecular species profiles of PC during different developing stages of tuber.
- figureS5.png
Supplementary figure 5: Composition and content analysis of unsatuated fatty acyl products in PC (A) and PE (B) during tuber developing stages.
- figureS5.png
Supplementary figure 5: Composition and content analysis of unsatuated fatty acyl products in PC (A) and PE (B) during tuber developing stages.
- Supplementaryfiledescription.docx
- Supplementaryfiledescription.docx
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Yuanyuan Dong
Jilin Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Oil crop varieties are currently subjected to an increasing worldwile demand and the tiger nut (Cyperus esculentus L.) attracts significant attention because of its huge capacity of lipids production. In eukaryotic cells, the balance between the accumulation of lipids and the distribution of certain pivotal molecules is fundamental for the regulation of many complex transcriptional regulatory networks. However, many studies have struggled to understand the dynamic of lipids and the transcriptomic mechanisms governing their biosynthesis and accumulation process during plant development.
Results: Our results displayed dynamic patterns for key lipids like glycolipid, phospholipid, and glyceride during the development of tiger nut tubers. Lipidomic analysis showed molecular species distribution of lipid class during developing stages. Here, we also characterrized transcription profiles of key transcripts that determined biosynthesis and distribution of natural lipids in tiger nuts tuber. The expression of FAD2 exhibited a significant influence on the molecular composition of phosphatidylcholines (PC) and phosphatidyl ethanolamine (PE) in tiger nuts. Moreover, during lipids accumulation, the expression pattern of three candidate transcripts of oleosin genes (OLE9, OLE10 and OLE11) also displayed significant leverage on the size of lipid drops.
Conclusion: We described the significant alterations in the composition of lipids class during developing stages of tiger nut tuber, we also revealed transcriptional profiles of genes invloed in lipid biosynthesis and accumulation. These results provided new landscapes for research on lipid composition, synthesis and accumulation during different developmental stages of plant tubers.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- FigureS1.jpg
Supplementary figure 1: Percentage of all molecular species of TAG in different developing stages.
- FigureS1.jpg
Supplementary figure 1: Percentage of all molecular species of TAG in different developing stages.
- FigureS2.jpg
Supplementary figure 2: Percentage of total molecular species of DG during five developing stages of tuber.
- FigureS2.jpg
Supplementary figure 2: Percentage of total molecular species of DG during five developing stages of tuber.
- FigureS3.jpg
Supplementary figure 3: Heatmap of all molecular species compotent of PE in five developing stages of tuber.
- FigureS3.jpg
Supplementary figure 3: Heatmap of all molecular species compotent of PE in five developing stages of tuber.
- FIgureS4.jpg
Supplementary figure 4: Compotent of total molecular species profiles of PC during different developing stages of tuber.
- FIgureS4.jpg
Supplementary figure 4: Compotent of total molecular species profiles of PC during different developing stages of tuber.
- figureS5.png
Supplementary figure 5: Composition and content analysis of unsatuated fatty acyl products in PC (A) and PE (B) during tuber developing stages.
- figureS5.png
Supplementary figure 5: Composition and content analysis of unsatuated fatty acyl products in PC (A) and PE (B) during tuber developing stages.
- Supplementaryfiledescription.docx
- Supplementaryfiledescription.docx
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