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Research Article
Sanjeev Kumar Singh
Alagappa University
Corresponding Author
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We announce the complete genome sequence of Bacillus tequilensis, a biosurfactant producing bacterium isolated from Chilika lake, Odisha, India(latitude and longitude: 19.8450 N 85.4788 E). The genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415 and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study we constructed a pathway which describe the biosurfactant metabolism of Bacillus tequilensis and identified the genes such as SrfAD, SrfAC, SrfAA which are involved in biosurfactant synthesis. The sequence of the same was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.
Keywords
Biosurfactant producing bacteria, Bacillus tequilensis, Genome sequence, Genome annotation, Next Generation Sequencing, De-novo assembly, Biosurfactant producing genes
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Sanjeev Kumar Singh
Alagappa University
Corresponding Author
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Complete author information
Appropriate declaration statements
Potential risks to human health
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History
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This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
We announce the complete genome sequence of Bacillus tequilensis, a biosurfactant producing bacterium isolated from Chilika lake, Odisha, India(latitude and longitude: 19.8450 N 85.4788 E). The genome sequence is 4.47 MB consisting of 4,478,749 base pairs forming a circular chromosome with 528 scaffolds, 4492 protein-encoding genes(ORFs), 81 tRNA genes, and 114 ribosomal RNA transcription units. The total number of raw reads was 4209415 and processed reads were 4058238 with predicted genes of 4492. The whole-genome obtained from the present investigation was used for genome annotation, variant calling, variant annotation and comparative genome analysis with other existing Bacillus species. In this study we constructed a pathway which describe the biosurfactant metabolism of Bacillus tequilensis and identified the genes such as SrfAD, SrfAC, SrfAA which are involved in biosurfactant synthesis. The sequence of the same was deposited in Genbank database with accession MUG02427.1, MUG02428.1, MUG02429.1, MUG03515.1 respectively. The whole-genome sequence was submitted to Genbank with an accession RMVO00000000 and the raw reads can be obtained from SRA, NCBI repository using accession: SRX5023292.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 4
Figure 4
Figure 5
Figure 5
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Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
Figure 9
Figure 9
Figure 10
Figure 10
Figure 11
Figure 11
Figure 12
Figure 12
Figure 13
Figure 13
Figure 14
Figure 14
This is a list of supplementary files associated with this preprint. Click to download.
- supplementarytitlepage.docx
- supplementarytitlepage.docx
- Supplementarytable1.xlsx
- Supplementarytable1.xlsx
- Supplementarytable2.xlsx
- Supplementarytable2.xlsx
- Supplementarytable3.xlsx
- Supplementarytable3.xlsx
- Supplementarytable5.xlsx
- Supplementarytable5.xlsx
- supplementarytitlepage.docx
- supplementarytitlepage.docx
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