Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates
Background: Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight, a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. While previous studies have shown F. poae isolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that there remain many secondary metabolites awaiting description.
Methods: We examined the biosynthetic potential of 38 F. poae isolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions.
Results: A high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase gene APS1 in approximately 7% of Eastern Canadian isolates surveyed.
Conclusions: Apicidin biosynthesis is linked to isolate-specific putative accessory chromosomes in F. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential of F. poae isolates.
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Due to technical limitations, table 1 is only available as a download in the Supplemental Files section.
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Posted 15 Dec, 2020
On 13 Jan, 2021
Received 23 Dec, 2020
Received 23 Dec, 2020
Received 23 Dec, 2020
Received 23 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
Invitations sent on 11 Dec, 2020
On 11 Dec, 2020
On 11 Dec, 2020
On 10 Dec, 2020
On 25 Nov, 2020
Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates
Posted 15 Dec, 2020
On 13 Jan, 2021
Received 23 Dec, 2020
Received 23 Dec, 2020
Received 23 Dec, 2020
Received 23 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
On 12 Dec, 2020
Invitations sent on 11 Dec, 2020
On 11 Dec, 2020
On 11 Dec, 2020
On 10 Dec, 2020
On 25 Nov, 2020
Background: Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight, a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. While previous studies have shown F. poae isolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that there remain many secondary metabolites awaiting description.
Methods: We examined the biosynthetic potential of 38 F. poae isolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions.
Results: A high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase gene APS1 in approximately 7% of Eastern Canadian isolates surveyed.
Conclusions: Apicidin biosynthesis is linked to isolate-specific putative accessory chromosomes in F. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential of F. poae isolates.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Due to technical limitations, table 1 is only available as a download in the Supplemental Files section.