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Research Article
Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates
David Overy
Agriculture and Agri-Food Canada
Corresponding Author
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Background: Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight, a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. While previous studies have shown F. poae isolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that there remain many secondary metabolites awaiting description.
Methods: We examined the biosynthetic potential of 38 F. poae isolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions.
Results: A high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase gene APS1 in approximately 7% of Eastern Canadian isolates surveyed.
Conclusions: Apicidin biosynthesis is linked to isolate-specific putative accessory chromosomes in F. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential of F. poae isolates.
Keywords
Fusarium poae, metabolomics, genomics, secondary metabolites, apicidin, fungal plant pathogens, mass spectrometry, accessory chromosomes, biosynthetic gene clusters
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Received 23 Dec, 2020
Reviewer #5 agreed
On 12 Dec, 2020
Reviewer #4 agreed
On 12 Dec, 2020
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On 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Reviewer #6 agreed
On 12 Dec, 2020
Reviewer #3 agreed
On 12 Dec, 2020
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On 11 Dec, 2020
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Apicidin Biosynthesis Is Linked to Accessory Chromosomes in Fusarium Poae Isolates
David Overy
Agriculture and Agri-Food Canada
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 15 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 13 Jan, 2021
Review #3 received
Received 23 Dec, 2020
Review #2 received
Received 23 Dec, 2020
Review #4 received
Received 23 Dec, 2020
Review #1 received
Received 23 Dec, 2020
Reviewer #5 agreed
On 12 Dec, 2020
Reviewer #4 agreed
On 12 Dec, 2020
Reviewer #2 agreed
On 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Reviewer #6 agreed
On 12 Dec, 2020
Reviewer #3 agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 11 Dec, 2020
Editor assigned
On 11 Dec, 2020
Editor invited
On 11 Dec, 2020
Submission checks complete
On 10 Dec, 2020
First submitted
On 25 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Fusarium poae is frequently associated with cereal crops showing symptoms of Fusarium head blight, a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination. While previous studies have shown F. poae isolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that there remain many secondary metabolites awaiting description.
Methods: We examined the biosynthetic potential of 38 F. poae isolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions.
Results: A high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase gene APS1 in approximately 7% of Eastern Canadian isolates surveyed.
Conclusions: Apicidin biosynthesis is linked to isolate-specific putative accessory chromosomes in F. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential of F. poae isolates.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Due to technical limitations, table 1 is only available as a download in the Supplemental Files section.