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Research Article
Protective effect of Calpain2 on mouse fibroblast L929 from injury induced by mechanical force
Li Hong
Wuhan University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Introduction and Hypothesis: To investigate the protective effect of Calpain2 on the mechanical force induced apoptosis in L929 cells, we compared the difference of Calpain2 in pelvic floor tissues between patients with uterine prolapse and those without uterine prolapse.
Methods: The cultured L929 cells were subjected to gene silencing treatment. The cells were grouped into control group, mechanical force group, sh-Capn2 group and sh-Capn2+ force group. Western blot and cell immunofluorescence assay were used to detect protein content: Calpain2, caspase3, Bcl2, Bax, cell apoptosis was detected by Annexin V-FITC. Each set of experiments was repeated three times.
Results: The expression of Calpain2 in pelvic floor tissues of patients with uterine prolapse was lower than that of patients without uterine prolapse. There was a negative correlation between Calpain2 and apoptosis, the decrease of Calpain2 was accompanied by the increase of apoptosis after exposing to mechanical force. There was no difference in Calpain2 expression between the sh-Capn2 group and the sh-Capn2 force group, but the latter had significant apoptosis, caspase3 was higher than the former, and the ratio of Bcl2/Bax was decreased.
Conclusions: The content of Calpain2 in pelvic floor tissue of normal and POP patients is different., and its expression has a protective effect on L929 cells from injury induced by mechanical force.
Keywords
POP, Mechanical force, Fibroblasts, Apoptosis
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CURRENT STATUS: Under Revision
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Editorial decision: Major revision
On 15 Jan, 2021
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Received 30 Dec, 2020
Reviewer #4 agreed
On 21 Dec, 2020
Reviewer #3 agreed
On 21 Dec, 2020
Reviewer #5 agreed
On 21 Dec, 2020
Reviewer #2 agreed
On 21 Dec, 2020
Reviewer #6 agreed
On 21 Dec, 2020
Reviewer #1 agreed
On 21 Dec, 2020
Reviewers invited
Invitations sent on 21 Dec, 2020
Editor assigned
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Protective effect of Calpain2 on mouse fibroblast L929 from injury induced by mechanical force
Li Hong
Wuhan University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 18 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 15 Jan, 2021
Review #2 received
Received 30 Dec, 2020
Review #1 received
Received 30 Dec, 2020
Review #3 received
Received 30 Dec, 2020
Review #4 received
Received 30 Dec, 2020
Reviewer #4 agreed
On 21 Dec, 2020
Reviewer #3 agreed
On 21 Dec, 2020
Reviewer #5 agreed
On 21 Dec, 2020
Reviewer #2 agreed
On 21 Dec, 2020
Reviewer #6 agreed
On 21 Dec, 2020
Reviewer #1 agreed
On 21 Dec, 2020
Reviewers invited
Invitations sent on 21 Dec, 2020
Editor assigned
On 17 Dec, 2020
Editor invited
On 17 Dec, 2020
Submission checks complete
On 17 Dec, 2020
First submitted
On 26 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Introduction and Hypothesis: To investigate the protective effect of Calpain2 on the mechanical force induced apoptosis in L929 cells, we compared the difference of Calpain2 in pelvic floor tissues between patients with uterine prolapse and those without uterine prolapse.
Methods: The cultured L929 cells were subjected to gene silencing treatment. The cells were grouped into control group, mechanical force group, sh-Capn2 group and sh-Capn2+ force group. Western blot and cell immunofluorescence assay were used to detect protein content: Calpain2, caspase3, Bcl2, Bax, cell apoptosis was detected by Annexin V-FITC. Each set of experiments was repeated three times.
Results: The expression of Calpain2 in pelvic floor tissues of patients with uterine prolapse was lower than that of patients without uterine prolapse. There was a negative correlation between Calpain2 and apoptosis, the decrease of Calpain2 was accompanied by the increase of apoptosis after exposing to mechanical force. There was no difference in Calpain2 expression between the sh-Capn2 group and the sh-Capn2 force group, but the latter had significant apoptosis, caspase3 was higher than the former, and the ratio of Bcl2/Bax was decreased.
Conclusions: The content of Calpain2 in pelvic floor tissue of normal and POP patients is different., and its expression has a protective effect on L929 cells from injury induced by mechanical force.
Figure 1
Figure 2
Figure 3
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.