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Research Article
Päivi Torkkeli
Dalhousie University
Corresponding Author
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Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families; the DEG/ENaC and TRP channels, and the TMC and Piezo proteins. We found twelve putative members of MET channel families in two spider transcriptomes, but detected only one, the Piezo protein, by in situ hybridization in their mechanosensory neurons. In contrast, probes for orthologs of TRP, ENaC or TMC genes that code MET channels in other species did not produce any signals in these cells. An antibody against C. salei Piezo detected the protein in all parts of their mechanosensory cells and in many neurons of the CNS. Unspecific blockers of MET channels, Ruthenium Red and GsMTx4, had no effect on the mechanically activated currents of the mechanosensory VS‑3 neurons, but the latter toxin reduced action potential firing when these cells were stimulated electrically. It is possible that the Piezo protein contributes to mechanosensory transduction in spider mechanosensilla, but it must have other functions in peripheral and central neurons.
Keywords
Mechanosensory neurons, mechanical forces, DEG/ENaC, TRP channels, Piezo proteins
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CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 09 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 20 Jan, 2021
Reviews received
Received 23 Dec, 2020
Reviewers agreed
On 22 Dec, 2020
Reviewers agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 01 Dec, 2020
Editor assigned
On 01 Dec, 2020
Editor invited
On 01 Dec, 2020
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On 27 Nov, 2020
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A new preprint design is coming!
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Päivi Torkkeli
Dalhousie University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 09 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 20 Jan, 2021
Reviews received
Received 23 Dec, 2020
Reviewers agreed
On 22 Dec, 2020
Reviewers agreed
On 11 Dec, 2020
Reviewers invited
Invitations sent on 01 Dec, 2020
Editor assigned
On 01 Dec, 2020
Editor invited
On 01 Dec, 2020
Submission checks complete
On 30 Nov, 2020
First submitted
On 27 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families; the DEG/ENaC and TRP channels, and the TMC and Piezo proteins. We found twelve putative members of MET channel families in two spider transcriptomes, but detected only one, the Piezo protein, by in situ hybridization in their mechanosensory neurons. In contrast, probes for orthologs of TRP, ENaC or TMC genes that code MET channels in other species did not produce any signals in these cells. An antibody against C. salei Piezo detected the protein in all parts of their mechanosensory cells and in many neurons of the CNS. Unspecific blockers of MET channels, Ruthenium Red and GsMTx4, had no effect on the mechanically activated currents of the mechanosensory VS‑3 neurons, but the latter toxin reduced action potential firing when these cells were stimulated electrically. It is possible that the Piezo protein contributes to mechanosensory transduction in spider mechanosensilla, but it must have other functions in peripheral and central neurons.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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