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Research article
Xuanchun Wang
Huashan Hospital Fudan University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2749-8497
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Heat shock protein 27 (HSP27) has been proposed as a vital protective factor in atherosclerosis. The objective of the present study was to evaluate the association between circulating HSP27 and intima–media thickness (IMT) of the common carotid artery in individuals with type 2 diabetes and to determine whether HSP27 represents an independent marker for subclinical atherosclerosis in this patient population.
Methods
We performed a cross-sectional community-based study consisting in 186 Chinese subjects with a median duration of diabetes of 8.2 years who underwent carotid ultrasound IMT measurement. Serum HSP27 levels were assessed by ELISA.
Results
Serum HSP27 levels were significantly higher in the IMT (+, >1.0 mm) group than in the IMT (-, ≤1.0 mm) group, with the median value of 8.80 ng/mL (5.62–12.25) and 6.93 ng/mL (4.23–9.60), respectively ( P =0.006). The discriminative value of HSP27 to evaluate IMT was 7.16 ng/mL and the area under the curve was 0.72 (95%CI=0.64–0.80, P=0.0065). Spearman's rank correlation analysis demonstrated that the concentrations of circulating HSP27 were positively correlated with carotid IMT ( r =0.198, P =0.007) and blood urea nitrogen ( r =0.170, P <0.05). Furthermore, in the logistic model, serum HSP27 levels were found to be independent predictors of carotid IMT in type 2 diabetic patients after adjustment for onset age of diabetes, blood pressure, total cholesterol and C-reactive protein. (OR=1.085, P =0.022).
Conclusions
Circulating HSP27 level, positively correlates with carotid IMT, is an independent predictor for early atherosclerotic changes in diabetes, and may represent a novel marker of subclinical atherosclerosis in type 2 diabetes.
Keywords
HSP27; carotid intima–media thickness; subclinical atherosclerosis; type 2 diabetes
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CURRENT STATUS: Published
View the published article at BMC Cardiovascular Disorders.
Version 3
Posted 30 Mar, 2020
No community comments so far
Editorial decision: Accept
On 27 Mar, 2020
Editor assigned
On 24 Mar, 2020
Submission checks complete
On 23 Mar, 2020
Editor invited
On 23 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 19 Mar, 2020
Reviewer #2 agreed
On 18 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 15 Mar, 2020
Editor assigned
On 12 Mar, 2020
Reviewers invited
Invitations sent on 12 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
Editor invited
On 11 Mar, 2020
No comments provided
Editorial decision: Major revision
On 09 Feb, 2020
Review #2 received
Received 08 Feb, 2020
Review #1 received
Received 26 Jan, 2020
Reviewer #2 agreed
On 25 Jan, 2020
Reviewers invited
Invitations sent on 21 Jan, 2020
Reviewer #1 agreed
On 21 Jan, 2020
Editor assigned
On 20 Jan, 2020
Submission checks complete
On 15 Jan, 2020
Editor invited
On 15 Jan, 2020
First submitted
On 14 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
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A new preprint design is coming!
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See the published version of this preprint at BMC Cardiovascular Disorders.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Xuanchun Wang
Huashan Hospital Fudan University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2749-8497
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cardiovascular Disorders.
Version 3
Posted 30 Mar, 2020
No community comments so far
Editorial decision: Accept
On 27 Mar, 2020
Editor assigned
On 24 Mar, 2020
Submission checks complete
On 23 Mar, 2020
Editor invited
On 23 Mar, 2020
No comments provided
Editorial decision: Minor revision
On 19 Mar, 2020
Reviewer #2 agreed
On 18 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 15 Mar, 2020
Editor assigned
On 12 Mar, 2020
Reviewers invited
Invitations sent on 12 Mar, 2020
Reviewer #1 agreed
On 12 Mar, 2020
Submission checks complete
On 11 Mar, 2020
Editor invited
On 11 Mar, 2020
No comments provided
Editorial decision: Major revision
On 09 Feb, 2020
Review #2 received
Received 08 Feb, 2020
Review #1 received
Received 26 Jan, 2020
Reviewer #2 agreed
On 25 Jan, 2020
Reviewers invited
Invitations sent on 21 Jan, 2020
Reviewer #1 agreed
On 21 Jan, 2020
Editor assigned
On 20 Jan, 2020
Submission checks complete
On 15 Jan, 2020
Editor invited
On 15 Jan, 2020
First submitted
On 14 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cardiovascular Disorders.
Background
Heat shock protein 27 (HSP27) has been proposed as a vital protective factor in atherosclerosis. The objective of the present study was to evaluate the association between circulating HSP27 and intima–media thickness (IMT) of the common carotid artery in individuals with type 2 diabetes and to determine whether HSP27 represents an independent marker for subclinical atherosclerosis in this patient population.
Methods
We performed a cross-sectional community-based study consisting in 186 Chinese subjects with a median duration of diabetes of 8.2 years who underwent carotid ultrasound IMT measurement. Serum HSP27 levels were assessed by ELISA.
Results
Serum HSP27 levels were significantly higher in the IMT (+, >1.0 mm) group than in the IMT (-, ≤1.0 mm) group, with the median value of 8.80 ng/mL (5.62–12.25) and 6.93 ng/mL (4.23–9.60), respectively ( P =0.006). The discriminative value of HSP27 to evaluate IMT was 7.16 ng/mL and the area under the curve was 0.72 (95%CI=0.64–0.80, P=0.0065). Spearman's rank correlation analysis demonstrated that the concentrations of circulating HSP27 were positively correlated with carotid IMT ( r =0.198, P =0.007) and blood urea nitrogen ( r =0.170, P <0.05). Furthermore, in the logistic model, serum HSP27 levels were found to be independent predictors of carotid IMT in type 2 diabetic patients after adjustment for onset age of diabetes, blood pressure, total cholesterol and C-reactive protein. (OR=1.085, P =0.022).
Conclusions
Circulating HSP27 level, positively correlates with carotid IMT, is an independent predictor for early atherosclerotic changes in diabetes, and may represent a novel marker of subclinical atherosclerosis in type 2 diabetes.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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