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Research article
Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation
Zhongmei Wen
Jilin University First Hospital
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: Listeria monocytogenes ( L. monocytogenes ) is a global opportunistic intracellular pathogen that can cause many diseases, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. The pore-forming toxin listeriolysin O (LLO) is one of the most important virulence factors of L. monocytogenes ; LLO can promote cytosolic bacterial proliferation and aid in evading the attacks of the host immune system. In addition, L. monocytogenes infection can trigger a series of severe inflammatory reactions.
Results: Here, on the basis of our previous studies, we further confirmed that morin lacking anti-Listeria activity could inhibit LLO oligomerization and found that morin could effectively alleviate the inflammation induced by Listeria in vivo and in vitro and has an obvious protective effect on infected cells and mice.
Conclusions: Thus, we further prove that morin is a valuable drug precursor to be developed for the treatment of Listeria infection.
Keywords
Listeria monocytogenes, listeriolysin O, morin, inflammation, anti-infection
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CURRENT STATUS: Under Review
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Posted 17 Apr, 2020
No community comments so far
Editorial decision: Minor revision
On 24 Apr, 2020
Review #3 received
Received 23 Apr, 2020
Review #2 received
Received 21 Apr, 2020
Reviewer #3 agreed
On 17 Apr, 2020
Review #1 received
Received 16 Apr, 2020
Reviewer #2 agreed
On 15 Apr, 2020
Reviewer #1 agreed
On 14 Apr, 2020
Editor assigned
On 14 Apr, 2020
Reviewers invited
Invitations sent on 14 Apr, 2020
Submission checks complete
On 13 Apr, 2020
Editor invited
On 13 Apr, 2020
No comments provided
Editorial decision: Major revision
On 12 Mar, 2020
Review #5 received
Received 10 Mar, 2020
Review #3 received
Received 21 Feb, 2020
Review #4 received
Received 21 Feb, 2020
Review #2 received
Received 14 Feb, 2020
Reviewer #5 agreed
On 13 Feb, 2020
Reviewer #2 agreed
On 11 Feb, 2020
Reviewer #3 agreed
On 11 Feb, 2020
Reviewer #4 agreed
On 11 Feb, 2020
Review #1 received
Received 11 Feb, 2020
Reviewer #1 agreed
On 30 Jan, 2020
Reviewers invited
Invitations sent on 29 Jan, 2020
Editor assigned
On 23 Jan, 2020
Submission checks complete
On 15 Jan, 2020
Editor invited
On 14 Jan, 2020
First submitted
On 06 Jan, 2020
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This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation
Zhongmei Wen
Jilin University First Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 17 Apr, 2020
No community comments so far
Editorial decision: Minor revision
On 24 Apr, 2020
Review #3 received
Received 23 Apr, 2020
Review #2 received
Received 21 Apr, 2020
Reviewer #3 agreed
On 17 Apr, 2020
Review #1 received
Received 16 Apr, 2020
Reviewer #2 agreed
On 15 Apr, 2020
Reviewer #1 agreed
On 14 Apr, 2020
Editor assigned
On 14 Apr, 2020
Reviewers invited
Invitations sent on 14 Apr, 2020
Submission checks complete
On 13 Apr, 2020
Editor invited
On 13 Apr, 2020
No comments provided
Editorial decision: Major revision
On 12 Mar, 2020
Review #5 received
Received 10 Mar, 2020
Review #3 received
Received 21 Feb, 2020
Review #4 received
Received 21 Feb, 2020
Review #2 received
Received 14 Feb, 2020
Reviewer #5 agreed
On 13 Feb, 2020
Reviewer #2 agreed
On 11 Feb, 2020
Reviewer #3 agreed
On 11 Feb, 2020
Reviewer #4 agreed
On 11 Feb, 2020
Review #1 received
Received 11 Feb, 2020
Reviewer #1 agreed
On 30 Jan, 2020
Reviewers invited
Invitations sent on 29 Jan, 2020
Editor assigned
On 23 Jan, 2020
Submission checks complete
On 15 Jan, 2020
Editor invited
On 14 Jan, 2020
First submitted
On 06 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Listeria monocytogenes ( L. monocytogenes ) is a global opportunistic intracellular pathogen that can cause many diseases, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. The pore-forming toxin listeriolysin O (LLO) is one of the most important virulence factors of L. monocytogenes ; LLO can promote cytosolic bacterial proliferation and aid in evading the attacks of the host immune system. In addition, L. monocytogenes infection can trigger a series of severe inflammatory reactions.
Results: Here, on the basis of our previous studies, we further confirmed that morin lacking anti-Listeria activity could inhibit LLO oligomerization and found that morin could effectively alleviate the inflammation induced by Listeria in vivo and in vitro and has an obvious protective effect on infected cells and mice.
Conclusions: Thus, we further prove that morin is a valuable drug precursor to be developed for the treatment of Listeria infection.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5