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Research article
Yan Feng
Fujian Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: "Core microbes" play a key role in the development of caries and lead to microflora disorders. Our goal was to detect the core microbes associated with the microbial flora imbalance in early childhood caries (ECC).
Methods: Fifteen caries-free children and fifteen high-caries (DMFT≥10) children aged 4-6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from plaque samples loaded in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.
Results: There were statistically significant differences between the groups regarding the relative abundance of S. mutans (Wilcoxon rank-sum test, P<0.05). No significant difference was found between the groups regarding other species or functional genes.
Conclusion: Neither unique microbes leading to early caries in deciduous teeth nor characteristics of a microbial community with a disordered structure were found. Some microorganisms related to dental caries in young children were found, but whether these microbes were truly related or were false positives requires further verification.
Keywords
Early childhood caries, Etiology, Oral microbiota, Microbial dysbiosis, 16S rRNA gene
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CURRENT STATUS: Published
View the published article at BMC Oral Health.
Version 1
Posted 02 Dec, 2020
No community comments so far
Editorial decision: Minor revision
On 17 Feb, 2021
Review #3 received
Received 16 Feb, 2021
Reviewer #3 agreed
On 30 Dec, 2020
Review #1 received
Received 29 Dec, 2020
Reviewer #2 agreed
On 29 Dec, 2020
Review #2 received
Received 29 Dec, 2020
Reviewers invited
Invitations sent on 14 Dec, 2020
Reviewer #1 agreed
On 14 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 28 Nov, 2020
Editor invited
On 23 Nov, 2020
First submitted
On 18 Nov, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
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A new preprint design is coming!
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See the published version of this preprint at BMC Oral Health.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Yan Feng
Fujian Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Oral Health.
Version 1
Posted 02 Dec, 2020
No community comments so far
Editorial decision: Minor revision
On 17 Feb, 2021
Review #3 received
Received 16 Feb, 2021
Reviewer #3 agreed
On 30 Dec, 2020
Review #1 received
Received 29 Dec, 2020
Reviewer #2 agreed
On 29 Dec, 2020
Review #2 received
Received 29 Dec, 2020
Reviewers invited
Invitations sent on 14 Dec, 2020
Reviewer #1 agreed
On 14 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 28 Nov, 2020
Editor invited
On 23 Nov, 2020
First submitted
On 18 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Oral Health.
Background: "Core microbes" play a key role in the development of caries and lead to microflora disorders. Our goal was to detect the core microbes associated with the microbial flora imbalance in early childhood caries (ECC).
Methods: Fifteen caries-free children and fifteen high-caries (DMFT≥10) children aged 4-6 years old were recruited according to the diagnostic criteria of caries suggested by the WHO. The 16S rRNA genes from plaque samples loaded in saliva were amplified by PCR, and the PCR products were sequenced by the Illumina Miseq platform. The sequencing results were analyzed by professional software to determine the composition and structure of the saliva microorganisms.
Results: There were statistically significant differences between the groups regarding the relative abundance of S. mutans (Wilcoxon rank-sum test, P<0.05). No significant difference was found between the groups regarding other species or functional genes.
Conclusion: Neither unique microbes leading to early caries in deciduous teeth nor characteristics of a microbial community with a disordered structure were found. Some microorganisms related to dental caries in young children were found, but whether these microbes were truly related or were false positives requires further verification.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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