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Li Qiu
West China Hospital of Sichuan University
Corresponding Author
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Background: Cancer urgently needs a new strategy for its recurrence and treatment resistance with the existing treatments. Fe-based metal-organic frameworks (MOFs) had the potential in ROS generation due to Fenton catalysis, which has been shown to be effective in antitumor therapy. However, Fenton catalysis requires sufficient H2O2 as the reactant to generate hydroxyl radical. Therefore, further improvement of Fe-MOF is needed. In the research, bio-/enzyme-mimics nanoparticles [email protected]@M were synthesized and were used combined with ultrasound targeted microbubble destruction (UTMD) as a novel method for cancer therapy.
Methods: [email protected]@M was synthesized by loading GOx and encapsulating tumor cell membrane. The nanostructures were characterized by SEM, TEM, XRD, EDS, XPS, and so on. The anti-tumor efficiency of [email protected]@M was evaluated by cytotoxicity test, live/dead cell staining and apoptosis ratio in vitro. The combination of [email protected]@M and UTMD was applied in vivo to verify the enhancement of anti-tumor effect of UTMD on [email protected]@M.
Results: [email protected]@M was successfully synthesized. [email protected]@M was most easily intake by A2780 tumor cells, generated the most ROS in tumor cells, and induced the most apoptotic tumor cells in vitro. In addition, UTMD technology further improved the anti-tumor efficiency in vivo due to its sonoporation, which helped create reversible holes in cell membranes for easier been destroyed by [email protected]@M.
Conclusion: Fe-MOF based bio-/enzyme-mimics nanoparticles [email protected]@M had excellent anti-tumor efficiency. And UTMD can improve the therapeutic effectiveness. The combination of [email protected]@M and UTMD provided a novel, safe, and efficient treatment strategy for cancer.
Keywords
Metal-organic frameworks, Ultrasound targeted microbubble destruction, cancer, Nanoparticles, Ultrasound, Treatment
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- Graphicalabstract11.20.docx
- Graphicalabstract11.20.docx
- SI11.19.docx
Supplementary information Supplementary information accompanies this paper at…..
- SI11.19.docx
Supplementary information Supplementary information accompanies this paper at…..
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View the published article at Journal of Nanobiotechnology.
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Editorial decision: Major revision
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Received 27 Dec, 2020
Review #1 received
Received 23 Dec, 2020
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On 13 Dec, 2020
Reviewer #2 agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
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On 27 Nov, 2020
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A new preprint design is coming!
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See the published version of this preprint at Journal of Nanobiotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Li Qiu
West China Hospital of Sichuan University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 1
Posted 04 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 29 Dec, 2020
Review #2 received
Received 27 Dec, 2020
Review #1 received
Received 23 Dec, 2020
Reviewer #3 agreed
On 13 Dec, 2020
Reviewer #2 agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 12 Dec, 2020
Reviewer #1 agreed
On 12 Dec, 2020
Editor invited
On 27 Nov, 2020
Editor assigned
On 27 Nov, 2020
Submission checks complete
On 27 Nov, 2020
First submitted
On 19 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Nanobiotechnology.
Background: Cancer urgently needs a new strategy for its recurrence and treatment resistance with the existing treatments. Fe-based metal-organic frameworks (MOFs) had the potential in ROS generation due to Fenton catalysis, which has been shown to be effective in antitumor therapy. However, Fenton catalysis requires sufficient H2O2 as the reactant to generate hydroxyl radical. Therefore, further improvement of Fe-MOF is needed. In the research, bio-/enzyme-mimics nanoparticles [email protected]@M were synthesized and were used combined with ultrasound targeted microbubble destruction (UTMD) as a novel method for cancer therapy.
Methods: [email protected]@M was synthesized by loading GOx and encapsulating tumor cell membrane. The nanostructures were characterized by SEM, TEM, XRD, EDS, XPS, and so on. The anti-tumor efficiency of [email protected]@M was evaluated by cytotoxicity test, live/dead cell staining and apoptosis ratio in vitro. The combination of [email protected]@M and UTMD was applied in vivo to verify the enhancement of anti-tumor effect of UTMD on [email protected]@M.
Results: [email protected]@M was successfully synthesized. [email protected]@M was most easily intake by A2780 tumor cells, generated the most ROS in tumor cells, and induced the most apoptotic tumor cells in vitro. In addition, UTMD technology further improved the anti-tumor efficiency in vivo due to its sonoporation, which helped create reversible holes in cell membranes for easier been destroyed by [email protected]@M.
Conclusion: Fe-MOF based bio-/enzyme-mimics nanoparticles [email protected]@M had excellent anti-tumor efficiency. And UTMD can improve the therapeutic effectiveness. The combination of [email protected]@M and UTMD provided a novel, safe, and efficient treatment strategy for cancer.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- Graphicalabstract11.20.docx
- Graphicalabstract11.20.docx
- SI11.19.docx
Supplementary information Supplementary information accompanies this paper at…..
- SI11.19.docx
Supplementary information Supplementary information accompanies this paper at…..
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