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Research
Laijin Lu
Jilin University First Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4244-7022
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Background: The primary strategy to repair peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Thus, the ability to direct and manipulate neuronal cell axon regeneration has been one of the top priorities in the field of neuroscience. A recent innovative approach for remotely guiding neuronal regeneration is to incorporate magnetic nanoparticles (MNPs) into cells and transfer the resulting MNP-loaded cells into a magnetically sensitive environment to respond to an external magnetic field. To realize this intention, the synthesis and preparation of ideal MNPs is an important challenge to overcome.
Results: In this study, we designed and prepared novel fluorescent-magnetic bifunctional Fe 3 O 4 ·Rhodamine [email protected] superparticles (FMSPs) as neural regeneration therapeutics. With the help of their excellent biocompatibility and ability to interact with neural cells, our in-house fabricated FMSPs can be endocytosed into cells, transported along the axons, and then aggregated in the growth cones. As a result, the mechanical forces generated by FMSPs can promote the growth and elongation of axons and stimulate gene expression associated with neuron growth under external magnetic fields.
Conclusions: Our work demonstrates that FMSPs can be used as a novel stimulator to promote noninvasive neural regeneration through cell magnetic actuation.
Keywords
Fe3O4 superparticles, magnetic nanoparticles, magnetic field, mechanical forces, magnetic actuation, axon regeneration, gene expression profile
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This is a list of supplementary files associated with this preprint. Click to download.
- TableofContentsEntry.tif
- Additionalfile4.MovieS3..mp4
- Additionalfile1.RevisionSupplementaryInformation..docx
- Additionalfile9.MovieS8..mp4
- Equations.docx
- Additionalfile8.MovieS7..mp4
- Additionalfile3.MovieS2..avi
- Additionalfile5.MovieS4..mp4
- Additionalfile6.MovieS5..mp4
- Additionalfile7.MovieS6..mp4
- Additionalfile2.MovieS1..avi
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CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 16 Apr, 2020
No community comments so far
Editorial decision: Accept
On 14 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
No comments provided
Editorial decision: Major revision
On 23 Mar, 2020
Review #1 received
Received 20 Mar, 2020
Review #2 received
Received 20 Mar, 2020
Reviewer #2 agreed
On 08 Mar, 2020
Reviewers invited
Invitations sent on 31 Jan, 2020
Reviewer #1 agreed
On 31 Jan, 2020
Editor invited
On 16 Jan, 2020
Editor assigned
On 16 Jan, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Nanobiotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Laijin Lu
Jilin University First Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4244-7022
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 16 Apr, 2020
No community comments so far
Editorial decision: Accept
On 14 Apr, 2020
Editor assigned
On 13 Apr, 2020
Submission checks complete
On 12 Apr, 2020
Editor invited
On 12 Apr, 2020
No comments provided
Editorial decision: Major revision
On 23 Mar, 2020
Review #1 received
Received 20 Mar, 2020
Review #2 received
Received 20 Mar, 2020
Reviewer #2 agreed
On 08 Mar, 2020
Reviewers invited
Invitations sent on 31 Jan, 2020
Reviewer #1 agreed
On 31 Jan, 2020
Editor invited
On 16 Jan, 2020
Editor assigned
On 16 Jan, 2020
First submitted
On 15 Jan, 2020
Submission checks complete
On 15 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Nanobiotechnology.
Background: The primary strategy to repair peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Thus, the ability to direct and manipulate neuronal cell axon regeneration has been one of the top priorities in the field of neuroscience. A recent innovative approach for remotely guiding neuronal regeneration is to incorporate magnetic nanoparticles (MNPs) into cells and transfer the resulting MNP-loaded cells into a magnetically sensitive environment to respond to an external magnetic field. To realize this intention, the synthesis and preparation of ideal MNPs is an important challenge to overcome.
Results: In this study, we designed and prepared novel fluorescent-magnetic bifunctional Fe 3 O 4 ·Rhodamine [email protected] superparticles (FMSPs) as neural regeneration therapeutics. With the help of their excellent biocompatibility and ability to interact with neural cells, our in-house fabricated FMSPs can be endocytosed into cells, transported along the axons, and then aggregated in the growth cones. As a result, the mechanical forces generated by FMSPs can promote the growth and elongation of axons and stimulate gene expression associated with neuron growth under external magnetic fields.
Conclusions: Our work demonstrates that FMSPs can be used as a novel stimulator to promote noninvasive neural regeneration through cell magnetic actuation.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
- TableofContentsEntry.tif
- Additionalfile4.MovieS3..mp4
- Additionalfile1.RevisionSupplementaryInformation..docx
- Additionalfile9.MovieS8..mp4
- Equations.docx
- Additionalfile8.MovieS7..mp4
- Additionalfile3.MovieS2..avi
- Additionalfile5.MovieS4..mp4
- Additionalfile6.MovieS5..mp4
- Additionalfile7.MovieS6..mp4
- Additionalfile2.MovieS1..avi
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