This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Chongbo Sun
ZheJiang Academy of Agricultural Sciences
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Sucrose is the primary form of photosynthetically produced carbohydrates transported long distance in many plant species, which significantly affects plant growth, development and physiology. Sucrose transporters (SUTs or SUCs) are a group of membrane proteins that play vital roles in mediating sucrose allocation within cells and at the whole plant level.
Results
In this study, we investigated the relationship of SUTs in 24 representative plant species and performed a comprehensive analysis of SUT genes in three sequenced Orchidaceae species, Dendrobium officinale, Phalaenopsis equestris, and Apostasia shenzhenica. All the SUTs from 24 plants were classified into three groups and five subgroups: subgroups A, B1, B2.1, B2.2, and C, based on the evolutionary relationships. A total of 22 SUT genes were identified in Orchidaceae species, among which D. officinale had 8 genes (DenSUT01-08), P. equestris had 8 genes (PeqSUT01-08) and A. shenzhenica had 6 genes (PeqSUT01-06). For the 22 Orchidaceae SUTs, each of the subgroups A, B2.2 and C contains three genes, whereas the SUT genes were significantly expanded in the monocot-specific subgroup B2.1 which contained 12 genes. To shed light into sucrose partitioning and functions of sucrose transporters in Orchidacea species, we analysed water-soluble sugar content and performed RNA sequencing of different tissues of D. officinale, including leaves, stems, flowers and roots. The results showed that although total content of water-soluble polysaccharides was highest in the stems of D. officinale, the sucrose content was highest in flowers. Moreover, gene expression analysis showed that most of the DenSUTs were expressed in flowers, among which DenSUT01, DenSUT07 and DenSUT06 had significantly high expression levels.
Conclusions
These results indicated that stems are used as main storage sinks for photosynthetically produced sugar in D. officinale, and that the DenSUTs mainly take functions in the cellular machinery and development of floral organs. Our findings provide valuable information on sucrose partitioning and the evolution and functions of SUT genes in Orchidaceae and other species.
Keywords
Sucrose transporters, Orchidaceae, gene family, water-soluble sugar content, gene expression
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.xlsx
Table S1 Protein sequence of the 22 SUTs from A. shenzhennica, D. officinale, and P. equestris.
- TableS2.xlsx
Table S2 FPKM expression of DenSUT genes in four different tissues of D. officinale.
- TableS3.xlsx
Table S3 Primer sequences used in qRT-PCR analysis.
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Chongbo Sun
ZheJiang Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 07 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Sucrose is the primary form of photosynthetically produced carbohydrates transported long distance in many plant species, which significantly affects plant growth, development and physiology. Sucrose transporters (SUTs or SUCs) are a group of membrane proteins that play vital roles in mediating sucrose allocation within cells and at the whole plant level.
Results
In this study, we investigated the relationship of SUTs in 24 representative plant species and performed a comprehensive analysis of SUT genes in three sequenced Orchidaceae species, Dendrobium officinale, Phalaenopsis equestris, and Apostasia shenzhenica. All the SUTs from 24 plants were classified into three groups and five subgroups: subgroups A, B1, B2.1, B2.2, and C, based on the evolutionary relationships. A total of 22 SUT genes were identified in Orchidaceae species, among which D. officinale had 8 genes (DenSUT01-08), P. equestris had 8 genes (PeqSUT01-08) and A. shenzhenica had 6 genes (PeqSUT01-06). For the 22 Orchidaceae SUTs, each of the subgroups A, B2.2 and C contains three genes, whereas the SUT genes were significantly expanded in the monocot-specific subgroup B2.1 which contained 12 genes. To shed light into sucrose partitioning and functions of sucrose transporters in Orchidacea species, we analysed water-soluble sugar content and performed RNA sequencing of different tissues of D. officinale, including leaves, stems, flowers and roots. The results showed that although total content of water-soluble polysaccharides was highest in the stems of D. officinale, the sucrose content was highest in flowers. Moreover, gene expression analysis showed that most of the DenSUTs were expressed in flowers, among which DenSUT01, DenSUT07 and DenSUT06 had significantly high expression levels.
Conclusions
These results indicated that stems are used as main storage sinks for photosynthetically produced sugar in D. officinale, and that the DenSUTs mainly take functions in the cellular machinery and development of floral organs. Our findings provide valuable information on sucrose partitioning and the evolution and functions of SUT genes in Orchidaceae and other species.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.xlsx
Table S1 Protein sequence of the 22 SUTs from A. shenzhennica, D. officinale, and P. equestris.
- TableS2.xlsx
Table S2 FPKM expression of DenSUT genes in four different tissues of D. officinale.
- TableS3.xlsx
Table S3 Primer sequences used in qRT-PCR analysis.
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