This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Primary research
MING XU
Jiangsu Province Center for Disease Control and Prevention
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8846-2880
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Acute myeloid leukemia (AML) is a common and lethal hematological malignant hyperplastic disease originating from hematopoietic stem cells. The purpose of this study is to obtain the key AML survival-related differentially expressed gene.
Methods: RNA sequencing (RNA-Seq) data and clinical information of patients were downloaded from the TCGA-LAML database. We focused the intersectional genes of survival-related DEGs, cytogenetics risk related-DEGs, and the top 10 pathways of both up- and down-regulated of Normalization Enrichment Score (NES). Multivariate Cox regression analyses were performed to analyze the independent factors for AML. The Kaplan–Meier and Nomogram analyses were plotted to predict and compare survival of AML patients. The validation of DEGs were performed by a clinical follow-up investigation.
Results: 151 RNA-Seq samples for 60,488 genes and 200 clinical samples were selected from the TCGA-database. After filtering with the conditions about survival-related DEGs, cytogenetics-risk associated, and predicted in top 10 pathways, IGHM was the only remaining gene. Cox analysis showed that IGHM expression and age displayed were intendent factors to the patients’ survival (P<0.05). Higher IGHM expression was identified in poor survival group (P<0.05), with 68%, 43%, and 30% in 1, 3, and 5-year survival investigation. GSEA analysis revealed that IGHM were mainly enriched in the immune response. In Chinese population, IGHM displayed its significance not in the childhood AML patients but in adult ones.
Conclusion: High expression of IHGM gene is an independent risky factor for the survival of patients with AML, which can be an important molecular marker for AML prognosis.
Keywords
Acute myeloid leukemia, TCGA, DEGs, Survival Analysis
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Primary research
MING XU
Jiangsu Province Center for Disease Control and Prevention
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8846-2880
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Acute myeloid leukemia (AML) is a common and lethal hematological malignant hyperplastic disease originating from hematopoietic stem cells. The purpose of this study is to obtain the key AML survival-related differentially expressed gene.
Methods: RNA sequencing (RNA-Seq) data and clinical information of patients were downloaded from the TCGA-LAML database. We focused the intersectional genes of survival-related DEGs, cytogenetics risk related-DEGs, and the top 10 pathways of both up- and down-regulated of Normalization Enrichment Score (NES). Multivariate Cox regression analyses were performed to analyze the independent factors for AML. The Kaplan–Meier and Nomogram analyses were plotted to predict and compare survival of AML patients. The validation of DEGs were performed by a clinical follow-up investigation.
Results: 151 RNA-Seq samples for 60,488 genes and 200 clinical samples were selected from the TCGA-database. After filtering with the conditions about survival-related DEGs, cytogenetics-risk associated, and predicted in top 10 pathways, IGHM was the only remaining gene. Cox analysis showed that IGHM expression and age displayed were intendent factors to the patients’ survival (P<0.05). Higher IGHM expression was identified in poor survival group (P<0.05), with 68%, 43%, and 30% in 1, 3, and 5-year survival investigation. GSEA analysis revealed that IGHM were mainly enriched in the immune response. In Chinese population, IGHM displayed its significance not in the childhood AML patients but in adult ones.
Conclusion: High expression of IHGM gene is an independent risky factor for the survival of patients with AML, which can be an important molecular marker for AML prognosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Loading...