DOI: https://doi.org/10.21203/rs.3.rs-120503/v1
Background
Long-chain polyunsaturated fatty acids (LCPUFA) are discussed to resolve chronic inflammation in asthma and other lung diseases. This study aimed at accelerating the incorporation kinetic of orally applied LCPUFA into lung tissue by co-applying medium-chain fatty acids (MCFA) which enhance the fat-metabolic rate.
Methods
Female C57BL/6 mice were orally supplemented with 700 mg eicosapentaenoic acid (EPA, C20:5n3) (group size: n=16) or additionally with 300 mg coconut oil with 4.2 wt% MCFA of total fat per kg body weight per day (group size: n=16) for 28 days. The fatty acid profile alterations of lung tissues were monitored by fatty acid methylester-analysis with capillary gaschromatography-flame-ionization detection over 63 days.
Results
The combined administration of EPA with MCFA increased its incorporation into the lung tissue up to day 28 (EPA 1.28 wt% ± 0,18; EPA and MCFA 1.83 wt% ± 0.17; Δ 0.55; p< 0.001 at day 28). But also its removal thereon was boosted compared to EPA supplementation alone (EPA 0.81 wt% ± 0.08; EPA and MCFA 0.58 wt% ± 0.06; Δ 0.23; p< 0.001 at day 31).
Conclusion
Based on the illustrated accelerating effects of MCFA on the incorporation and removal kinetics of dietary LCPUFA into lung tissue, the present study suggests a two-phase strategy for immune-regulative dietary LCPUFA-supplementation. At the very beginning supplementations should be accompanied with MCFA to support the LCPUFA-uptake, thereon MCFA should be omitted to stabilize the LCPUFA-status in the body.
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