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Research article
Yong-Fu Fu
Institute of Crop Science, Chinese Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1486-0146
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Starch is synthesized during daylight for temporary storage in leaves and then degraded during the subsequent night to support plant growth and development. Impairment of starch degradation leads to stunted growth, even senescence and death. The nuclear pore complex is involved in many cellular processes, but its relationship with starch degradation has been unclear until now. We previously identified that two Nucleoporin98 genes (Nup98a and Nup98b) redundantly regulate flowering via the CONSTANS (CO)-independent pathway in Arabidopsis thaliana. The double mutant also shows severe senescence phenotypes.
Results: We find that Nucleoporin 98 participates in the regulation of sugar metabolism in leaves and is also involved in senescence regulation in Arabidopsis. We show that Nup98a and Nup98b function redundantly at different stages of starch degradation. The nup98a-1 nup98b-1 double mutant accumulates more starch, showing a severe early senescence phenotype compared to wild type plants. The expression of marker genes related to starch degradation is impaired in the nup98a-1 nup98b-1 double mutant, and marker genes of carbon starvation and senescence express their products earlier and in higher abundance than in wild type plants, suggesting that abnormalities in energy metabolism are the main cause of senescence in the double mutant. Addition of sucrose to the growth medium rescues early senescence phenotypes of the nup98a-1 nup98b-1 mutant.
Conclusions: Our results provide evidence for a novel role of the nuclear pore complex in energy metabolism related to growth and development, in which Nup98 functions in starch degradation to control growth regulation in Arabidopsis.
Keywords
Nup98; Starch; Sugar; Senescence; Nuclear pore complex; Nucleoporin.
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View the published article at BMC Plant Biology.
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Editorial decision: Minor revision
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On 24 May, 2020
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Review #2 received
Received 13 May, 2020
Editorial decision: Minor revision
On 13 May, 2020
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Received 04 May, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 25 Apr, 2020
Reviewers invited
Invitations sent on 23 Apr, 2020
Editor assigned
On 21 Apr, 2020
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On 20 Apr, 2020
Editor invited
On 20 Apr, 2020
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Editorial decision: Major revision
On 01 Mar, 2020
Review #2 received
Received 29 Feb, 2020
Reviewer #2 agreed
On 10 Feb, 2020
Review #1 received
Received 01 Feb, 2020
Reviewer #1 agreed
On 20 Jan, 2020
Editor assigned
On 17 Jan, 2020
Reviewers invited
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Subject Areas
Plant Molecular Biology and Genetics
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Yong-Fu Fu
Institute of Crop Science, Chinese Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1486-0146
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
Version 5
Posted 13 Jun, 2020
No community comments so far
Editorial decision: Accept
On 12 Jun, 2020
Editor assigned
On 10 Jun, 2020
Submission checks complete
On 09 Jun, 2020
Editor invited
On 09 Jun, 2020
No comments provided
Submission checks complete
On 02 Jun, 2020
Editorial decision: Minor revision
On 01 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 27 May, 2020
Editor assigned
On 24 May, 2020
Submission checks complete
On 23 May, 2020
Editor invited
On 23 May, 2020
No comments provided
Review #2 received
Received 13 May, 2020
Editorial decision: Minor revision
On 13 May, 2020
Review #1 received
Received 04 May, 2020
Reviewer #2 agreed
On 25 Apr, 2020
Reviewer #1 agreed
On 25 Apr, 2020
Reviewers invited
Invitations sent on 23 Apr, 2020
Editor assigned
On 21 Apr, 2020
Submission checks complete
On 20 Apr, 2020
Editor invited
On 20 Apr, 2020
No comments provided
Editorial decision: Major revision
On 01 Mar, 2020
Review #2 received
Received 29 Feb, 2020
Reviewer #2 agreed
On 10 Feb, 2020
Review #1 received
Received 01 Feb, 2020
Reviewer #1 agreed
On 20 Jan, 2020
Editor assigned
On 17 Jan, 2020
Reviewers invited
Invitations sent on 17 Jan, 2020
Submission checks complete
On 17 Jan, 2020
Editor invited
On 17 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background: Starch is synthesized during daylight for temporary storage in leaves and then degraded during the subsequent night to support plant growth and development. Impairment of starch degradation leads to stunted growth, even senescence and death. The nuclear pore complex is involved in many cellular processes, but its relationship with starch degradation has been unclear until now. We previously identified that two Nucleoporin98 genes (Nup98a and Nup98b) redundantly regulate flowering via the CONSTANS (CO)-independent pathway in Arabidopsis thaliana. The double mutant also shows severe senescence phenotypes.
Results: We find that Nucleoporin 98 participates in the regulation of sugar metabolism in leaves and is also involved in senescence regulation in Arabidopsis. We show that Nup98a and Nup98b function redundantly at different stages of starch degradation. The nup98a-1 nup98b-1 double mutant accumulates more starch, showing a severe early senescence phenotype compared to wild type plants. The expression of marker genes related to starch degradation is impaired in the nup98a-1 nup98b-1 double mutant, and marker genes of carbon starvation and senescence express their products earlier and in higher abundance than in wild type plants, suggesting that abnormalities in energy metabolism are the main cause of senescence in the double mutant. Addition of sucrose to the growth medium rescues early senescence phenotypes of the nup98a-1 nup98b-1 mutant.
Conclusions: Our results provide evidence for a novel role of the nuclear pore complex in energy metabolism related to growth and development, in which Nup98 functions in starch degradation to control growth regulation in Arabidopsis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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