The construction of water conservancy projects has changed the hydrological situation of rivers and has an essential impact on the river ecosystem. The influence modes of different factors on runoff alteration are discussed to improve the development and utilization of water resources and promote ecological benefits. The ecological, hydrological index change range method (IHA-RVA) and hydrological alteration degree method were comprehensively used to evaluate Min River's hydrological situation. Based on six budyko hypothesis formulas, the contribution rates of climate change and human activities to runoff change are quantitatively analyzed. The study showed that the runoff of Min River basin showed a significant decreasing trend from 1960 to 2019 and a sudden alteration around 1993; The overall alteration in runoff conditions was 45% moderate, and the overall alteration in precipitation was 37% moderate; Precipitation and potential evapotranspiration also showed a decreasing trend within the same period, but the overall trend was not significant; The contribution of climate alteration to runoff alteration is 30.2%, and the contribution of human activities to runoff alteration is 69.8%, human activities are the dominant factor affecting the alteration of runoff situation in Min River basin.