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Research Article
Chanabasayya Vastrad
Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karnataka, India.
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Obesity associated type 2 diabetes mellitus is a metabolic disorder ; however, the etiology of obesity associated type 2 diabetes mellitus remains largely unknown. There is an urgent need to further broaden the understanding of the development mechanism of obesity associated type 2 diabetes mellitus.
Methods
To screen the differentially expressed genes (DEGs) that may play essential roles in obesity associated type 2 diabetes mellitus, the public expression profiling by high throughput sequencing data (GSE143319) were downloaded and screened for DEGs. Then, Gene Ontology (GO) function analysis and REACTOME pathway analysis were performed. To screen hub and target genes, the protein–protein interaction network, miRNA-target genes regulatory network and TF-target gene regulatory network were constructed. The Receiver operating characteristic (ROC) curve analysis and RT- PCR analysis of hub genes in obesity associated type 2 diabetes mellitus were also analyzed. Final molecular docking studies performed for screening small drug molecules.
Results
There were 409 up regulated and 411 down regulated genes detected, and the biological processes of the GO analysis were enriched in regulation of ion transmembrane transport, intrinsic component of plasma membrane, transferase activity, transferring phosphorus-containing groups, cell adhesion, integral component of plasma membrane and signaling receptor binding, whereas, the REACTOME pathway analysis was enriched in integration of energy metabolism and extracellular matrix organization. The hub genes CEBPD, TP73, ESR2, TAB1, MAP3K5, FN1, UBD, RUNX1, PIK3R2 and TNF, which might play a essential role in obesity associated type 2 diabetes mellitus was further screened.
Conclusions
The present study could deepen the understanding of the molecular mechanism of obesity associated type 2 diabetes mellitus, which could be useful in developing clinical treatments of obesity associated type 2 diabetes mellitus.
Keywords
obesity associated type 2 diabetes mellitus, differentially expressed gene, pathway, protein-protein interaction network, miRNA-target genes regulatory network
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CURRENT STATUS: Published
View the published article at BMC Endocrine Disorders.
Version 1
Posted 11 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 18 Jan, 2021
Reviews received
Received 28 Dec, 2020
Reviewers agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 10 Dec, 2020
Editor assigned
On 07 Dec, 2020
Editor invited
On 07 Dec, 2020
Submission checks complete
On 07 Dec, 2020
First submitted
On 04 Dec, 2020
Metrics
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PDF Downloads: ...
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Subject Areas
Endocrinology & Metabolism
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Endocrine Disorders.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Chanabasayya Vastrad
Biostatistics and Bioinformatics, Chanabasava Nilaya, Bharthinagar, Dharwad 580001, Karnataka, India.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Endocrine Disorders.
Version 1
Posted 11 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 18 Jan, 2021
Reviews received
Received 28 Dec, 2020
Reviewers agreed
On 12 Dec, 2020
Reviewers invited
Invitations sent on 10 Dec, 2020
Editor assigned
On 07 Dec, 2020
Editor invited
On 07 Dec, 2020
Submission checks complete
On 07 Dec, 2020
First submitted
On 04 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Endocrinology & Metabolism
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Endocrine Disorders.
Background
Obesity associated type 2 diabetes mellitus is a metabolic disorder ; however, the etiology of obesity associated type 2 diabetes mellitus remains largely unknown. There is an urgent need to further broaden the understanding of the development mechanism of obesity associated type 2 diabetes mellitus.
Methods
To screen the differentially expressed genes (DEGs) that may play essential roles in obesity associated type 2 diabetes mellitus, the public expression profiling by high throughput sequencing data (GSE143319) were downloaded and screened for DEGs. Then, Gene Ontology (GO) function analysis and REACTOME pathway analysis were performed. To screen hub and target genes, the protein–protein interaction network, miRNA-target genes regulatory network and TF-target gene regulatory network were constructed. The Receiver operating characteristic (ROC) curve analysis and RT- PCR analysis of hub genes in obesity associated type 2 diabetes mellitus were also analyzed. Final molecular docking studies performed for screening small drug molecules.
Results
There were 409 up regulated and 411 down regulated genes detected, and the biological processes of the GO analysis were enriched in regulation of ion transmembrane transport, intrinsic component of plasma membrane, transferase activity, transferring phosphorus-containing groups, cell adhesion, integral component of plasma membrane and signaling receptor binding, whereas, the REACTOME pathway analysis was enriched in integration of energy metabolism and extracellular matrix organization. The hub genes CEBPD, TP73, ESR2, TAB1, MAP3K5, FN1, UBD, RUNX1, PIK3R2 and TNF, which might play a essential role in obesity associated type 2 diabetes mellitus was further screened.
Conclusions
The present study could deepen the understanding of the molecular mechanism of obesity associated type 2 diabetes mellitus, which could be useful in developing clinical treatments of obesity associated type 2 diabetes mellitus.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
Figure 9
Figure 9
Figure 10
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
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