This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research note
FOLUKE Adedayo AKANDE
Federal University of Agriculture Abeokuta College of Veterinary Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8740-8492
License:
This work is licensed under a CC BY 4.0 License. Read Full License
OBJECTIVES : Cattle are important livestock species in Nigeria. But disease agents, especially gastrointestinal (GI) parasites, hinder their productivity. Non availability of rapid and affordable tools for GI parasite diagnosis poses problem to practitioners thus, the use of rapid, highly sensitive diagnostic techniques is required. One hundred faecal samples from cattle were analyzed through Simple Faecal Flotation (SFF), Modified Centrifugal Faecal Flotation (MCFF) and Mini-FLOTAC ® techniques (MFT) using Sodium Chloride, Brine, Sugar, Salt/Sugar and Zinc Sulphate at specific gravity of 1.2, 1.23, 1.25, 1.3 and 1.3 respectively as Flotation Solutions (FS).
RESULTS : 100% GI parasite prevalence of was recorded with the following distribution: Strongyle (99%), Strongyloides spp (97%), Neoascaris vitulorum (78%), Trichostrongylus (56%), Nematodirus (46%), Capillaria spp (14%) and Trichuris spp (6%), Moniezia benedini (24%), Moniezia expansa (16%) , Taenia-like egg (3%), Schistosoma eggs (3%) and Eimeria oocyst (100%). Using Salt/Sugar the sensitivity of the techniques was 61.99%, 58.49% and 54.24%. for MFT, SFF and MCFF respectively. Considering the sensitivity of Salt/Sugar FS with availability and affordability its use as a routine FS is advocated. The ease and rapid use of Mini-FLOTAC in low economy country in addition to safety of diagnostic staff and the possibility of re-usage.
Keywords
Cattle, copro-analysis, Mini-FLOTAC®, parasite elements, Salt/Sugar
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research note
FOLUKE Adedayo AKANDE
Federal University of Agriculture Abeokuta College of Veterinary Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8740-8492
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Jan, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
OBJECTIVES : Cattle are important livestock species in Nigeria. But disease agents, especially gastrointestinal (GI) parasites, hinder their productivity. Non availability of rapid and affordable tools for GI parasite diagnosis poses problem to practitioners thus, the use of rapid, highly sensitive diagnostic techniques is required. One hundred faecal samples from cattle were analyzed through Simple Faecal Flotation (SFF), Modified Centrifugal Faecal Flotation (MCFF) and Mini-FLOTAC ® techniques (MFT) using Sodium Chloride, Brine, Sugar, Salt/Sugar and Zinc Sulphate at specific gravity of 1.2, 1.23, 1.25, 1.3 and 1.3 respectively as Flotation Solutions (FS).
RESULTS : 100% GI parasite prevalence of was recorded with the following distribution: Strongyle (99%), Strongyloides spp (97%), Neoascaris vitulorum (78%), Trichostrongylus (56%), Nematodirus (46%), Capillaria spp (14%) and Trichuris spp (6%), Moniezia benedini (24%), Moniezia expansa (16%) , Taenia-like egg (3%), Schistosoma eggs (3%) and Eimeria oocyst (100%). Using Salt/Sugar the sensitivity of the techniques was 61.99%, 58.49% and 54.24%. for MFT, SFF and MCFF respectively. Considering the sensitivity of Salt/Sugar FS with availability and affordability its use as a routine FS is advocated. The ease and rapid use of Mini-FLOTAC in low economy country in addition to safety of diagnostic staff and the possibility of re-usage.
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