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Research Article
Ana López-Malvar
University of Vigo
Corresponding Author
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Background: Mechanical resistance due to higher hydroxycinnamate content makes maize tissues more recalcitrant to damage by insects, less digestible by ruminants, and less suitable for biofuel production. The integrated study of the maize functional genetic variability for each hydroxycinnamate component could be crucial to identify relevant genetic variants that may be incorporated into selection programs to breed maize varieties for multiple uses. A Genome Wide Association study was carried out a in a maize Multiparent-Advanced Intercross (MAGIC) Population to indentify Single Nucleotide Polymorphisms (SNPs) associated with cell wall bound hydroxycinnamates;and we checked thereafter their relationship with SNPs significantly associated with saccharification efficiency, digestibility of organic matter and corn borer damage.
Results: We found 24 SNPs, corresponding to15 QTL, significantly associated with cell wall bound hydroxycinnamates. Each SNP explained between 6 and 8% of the total variability. We define new genomic regions and genes involved in polysaccharide synthesis and modifications, and the oxidative coupling associatted to cell wall hydroxycinnamates content.
Conclusions: SNPs explained a small proportion of the variability for hydroxycinnamates, saccharification efficiency, digestibility or insect damage, therefore we recommend a genomic selection approach for future breeding programs of these traits. In addition, no colocalizations were found between hydroxycinnamates and final-use-related traits so breeding strategies can be focus on each particular trait with no side effects on the others.
Keywords
Zea mays, MAGIC, GWAS, cell wall hydroxycinnamates, ethanol, digestibility
This is a list of supplementary files associated with this preprint. Click to download.
- SuppelmentaryFigure1.docx
Supplementary Figure 1: Quantile-quantile plots of the GWAS mixed linear model for cell wall bound hydroxycinnamates in a maize multiparent advanced-generation intercross population.
- SuppelmentaryFigure1.docx
Supplementary Figure 1: Quantile-quantile plots of the GWAS mixed linear model for cell wall bound hydroxycinnamates in a maize multiparent advanced-generation intercross population.
- SuppelmentaryTable1.docx
Supplementary Table 1: Complete list of candidate genes for QTLs found for Cell Wall Component bound Hydroxycinnamates in a MAGIC population.
- SuppelmentaryTable1.docx
Supplementary Table 1: Complete list of candidate genes for QTLs found for Cell Wall Component bound Hydroxycinnamates in a MAGIC population.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Ana López-Malvar
University of Vigo
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Mechanical resistance due to higher hydroxycinnamate content makes maize tissues more recalcitrant to damage by insects, less digestible by ruminants, and less suitable for biofuel production. The integrated study of the maize functional genetic variability for each hydroxycinnamate component could be crucial to identify relevant genetic variants that may be incorporated into selection programs to breed maize varieties for multiple uses. A Genome Wide Association study was carried out a in a maize Multiparent-Advanced Intercross (MAGIC) Population to indentify Single Nucleotide Polymorphisms (SNPs) associated with cell wall bound hydroxycinnamates;and we checked thereafter their relationship with SNPs significantly associated with saccharification efficiency, digestibility of organic matter and corn borer damage.
Results: We found 24 SNPs, corresponding to15 QTL, significantly associated with cell wall bound hydroxycinnamates. Each SNP explained between 6 and 8% of the total variability. We define new genomic regions and genes involved in polysaccharide synthesis and modifications, and the oxidative coupling associatted to cell wall hydroxycinnamates content.
Conclusions: SNPs explained a small proportion of the variability for hydroxycinnamates, saccharification efficiency, digestibility or insect damage, therefore we recommend a genomic selection approach for future breeding programs of these traits. In addition, no colocalizations were found between hydroxycinnamates and final-use-related traits so breeding strategies can be focus on each particular trait with no side effects on the others.
This is a list of supplementary files associated with this preprint. Click to download.
- SuppelmentaryFigure1.docx
Supplementary Figure 1: Quantile-quantile plots of the GWAS mixed linear model for cell wall bound hydroxycinnamates in a maize multiparent advanced-generation intercross population.
- SuppelmentaryFigure1.docx
Supplementary Figure 1: Quantile-quantile plots of the GWAS mixed linear model for cell wall bound hydroxycinnamates in a maize multiparent advanced-generation intercross population.
- SuppelmentaryTable1.docx
Supplementary Table 1: Complete list of candidate genes for QTLs found for Cell Wall Component bound Hydroxycinnamates in a MAGIC population.
- SuppelmentaryTable1.docx
Supplementary Table 1: Complete list of candidate genes for QTLs found for Cell Wall Component bound Hydroxycinnamates in a MAGIC population.
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