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Research Article
Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster
Olga Kyrchanova
Institute of Gene Biology
Corresponding Author
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The autonomy of segment-specific regulatory domains in the Bithorax complex is conferred by boundary elements and associated Polycomb response elements (PREs). The Fab-6 boundary is located at the junction of the iab-5 and iab-6 domains. Previous studies mapped it to a nuclease hypersensitive region 1 (HS1), while the iab-6 PRE was mapped to a second hypersensitive region HS2 nearly 3 kb away. To analyze the role of HS1 and HS2 in boundary we generated deletions of HS1 or HS1 + HS2 that have attP site for boundary replacement experiments. The 1389 bp HS1 deletion can be rescued by a 529 bp core Fab-6 sequence that includes two CTCF sites. However, Fab-6 HS1 cannot rescue the HS1 + HS2 deletion or substitute for another BX-C boundary – Fab-7. For this it must be combined with a PRE, either Fab-7 HS3, or Fab-6 HS2. These findings suggest that the boundary function of Fab-6 HS1 must be bolstered by an element that has PRE activity.
Keywords
CTCF, chromatin boundary, architectural proteins, PRE, bithorax
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster
Olga Kyrchanova
Institute of Gene Biology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 16 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 12 Jan, 2021
Review #3 received
Received 31 Dec, 2020
Review #1 received
Received 31 Dec, 2020
Review #5 received
Received 31 Dec, 2020
Review #6 received
Received 31 Dec, 2020
Review #4 received
Received 31 Dec, 2020
Review #2 received
Received 31 Dec, 2020
Reviewer #4 agreed
On 18 Dec, 2020
Reviewer #5 agreed
On 18 Dec, 2020
Reviewer #12 agreed
On 16 Dec, 2020
Reviewer #6 agreed
On 16 Dec, 2020
Reviewer #10 agreed
On 16 Dec, 2020
Reviewer #8 agreed
On 16 Dec, 2020
Reviewer #2 agreed
On 16 Dec, 2020
Reviewer #1 agreed
On 16 Dec, 2020
Reviewer #3 agreed
On 16 Dec, 2020
Reviewer #9 agreed
On 16 Dec, 2020
Reviewer #7 agreed
On 16 Dec, 2020
Reviewer #14 agreed
On 16 Dec, 2020
Reviewer #15 agreed
On 16 Dec, 2020
Reviewer #13 agreed
On 16 Dec, 2020
Reviewer #11 agreed
On 16 Dec, 2020
Reviewers invited
Invitations sent on 15 Dec, 2020
Editor assigned
On 15 Dec, 2020
Editor invited
On 15 Dec, 2020
Submission checks complete
On 14 Dec, 2020
First submitted
On 04 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The autonomy of segment-specific regulatory domains in the Bithorax complex is conferred by boundary elements and associated Polycomb response elements (PREs). The Fab-6 boundary is located at the junction of the iab-5 and iab-6 domains. Previous studies mapped it to a nuclease hypersensitive region 1 (HS1), while the iab-6 PRE was mapped to a second hypersensitive region HS2 nearly 3 kb away. To analyze the role of HS1 and HS2 in boundary we generated deletions of HS1 or HS1 + HS2 that have attP site for boundary replacement experiments. The 1389 bp HS1 deletion can be rescued by a 529 bp core Fab-6 sequence that includes two CTCF sites. However, Fab-6 HS1 cannot rescue the HS1 + HS2 deletion or substitute for another BX-C boundary – Fab-7. For this it must be combined with a PRE, either Fab-7 HS3, or Fab-6 HS2. These findings suggest that the boundary function of Fab-6 HS1 must be bolstered by an element that has PRE activity.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5