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Research article
Massoud MIRSHAHI
Corresponding Author
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Background: It may be impossible to perform cancer surgery with free margins in the presence of an unresectable structure. Local drug treatment after surgery has been proposed to increase the rate of tumor control.
Methods: Multi-nanolayers (10-330nm) were generated by a low-pressure (375mTorr) inductively coupled plasma (13.56MHz) reactor for anticancer drug delivery by the deposition of polycaprolactone-polyethylene glycol multistack barrier on the collagen membrane (100µm thickness). Carboplatin (300µg/cm 2 ) was used for the in vitro and in vivo investigations. Energy-dispersive X-ray spectroscopy (15keV), scanning electron microscopy and inductively coupled plasma mass spectrometry were used to detect the presence of carboplatin in the nanolayer, the tumor sample and the culture medium. Preclinical studies were performed on ovarian (OVCAR-3NIH) and colon (CT26) cancer cell lines as xenografts (45 days) and allografts (23 days) in Swiss-nude (n=6) and immunocompetent BALB/cByJ mice (n=24), respectively.
Results: The loading of carboplatin or other drugs between the nanofilm on the collagen membrane did not modify the mesh complex architecture or the drug properties. Drugs were detectable on the membrane for more than two weeks in the in vitro analysis and more than 10 days in the in vivo analysis. Cytotoxic mesh decreased cell adherence (down 5.42-fold) and induced cancer cell destruction (up to 7.87-fold). Implantation of the mesh on the mouse tumor nodule modified the cell architecture and decreased the tumor size (50.26%) compared to the control by inducing cell apoptosis.
Conclusion: Plasma technology allows a mesh to be built with multi-nanolayer anticancer drug delivery on collagen membranes.
Keywords
Cancer, drug delivery, film implantation, Multi-nanolayer technology, Radio Frequency Plasma
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CURRENT STATUS: Published
View the published article at BMC Cancer.
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Posted 22 Jan, 2020
No community comments so far
Editorial decision: Major revision
On 20 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 07 Mar, 2020
Reviewer #2 agreed
On 05 Mar, 2020
Reviewers invited
Invitations sent on 03 Mar, 2020
Reviewer #1 agreed
On 03 Mar, 2020
Editor assigned
On 27 Jan, 2020
Submission checks complete
On 21 Jan, 2020
Editor invited
On 20 Jan, 2020
First submitted
On 15 Jan, 2020
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See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Massoud MIRSHAHI
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 1
Posted 22 Jan, 2020
No community comments so far
Editorial decision: Major revision
On 20 Mar, 2020
Review #2 received
Received 18 Mar, 2020
Review #1 received
Received 07 Mar, 2020
Reviewer #2 agreed
On 05 Mar, 2020
Reviewers invited
Invitations sent on 03 Mar, 2020
Reviewer #1 agreed
On 03 Mar, 2020
Editor assigned
On 27 Jan, 2020
Submission checks complete
On 21 Jan, 2020
Editor invited
On 20 Jan, 2020
First submitted
On 15 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background: It may be impossible to perform cancer surgery with free margins in the presence of an unresectable structure. Local drug treatment after surgery has been proposed to increase the rate of tumor control.
Methods: Multi-nanolayers (10-330nm) were generated by a low-pressure (375mTorr) inductively coupled plasma (13.56MHz) reactor for anticancer drug delivery by the deposition of polycaprolactone-polyethylene glycol multistack barrier on the collagen membrane (100µm thickness). Carboplatin (300µg/cm 2 ) was used for the in vitro and in vivo investigations. Energy-dispersive X-ray spectroscopy (15keV), scanning electron microscopy and inductively coupled plasma mass spectrometry were used to detect the presence of carboplatin in the nanolayer, the tumor sample and the culture medium. Preclinical studies were performed on ovarian (OVCAR-3NIH) and colon (CT26) cancer cell lines as xenografts (45 days) and allografts (23 days) in Swiss-nude (n=6) and immunocompetent BALB/cByJ mice (n=24), respectively.
Results: The loading of carboplatin or other drugs between the nanofilm on the collagen membrane did not modify the mesh complex architecture or the drug properties. Drugs were detectable on the membrane for more than two weeks in the in vitro analysis and more than 10 days in the in vivo analysis. Cytotoxic mesh decreased cell adherence (down 5.42-fold) and induced cancer cell destruction (up to 7.87-fold). Implantation of the mesh on the mouse tumor nodule modified the cell architecture and decreased the tumor size (50.26%) compared to the control by inducing cell apoptosis.
Conclusion: Plasma technology allows a mesh to be built with multi-nanolayer anticancer drug delivery on collagen membranes.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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