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Research Article
Lin Meng
Beijing Academy of Agriculture and Forestry Sciences
Corresponding Author
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As an important nutrient, K+ plays a crucial role in plant stress resistance. It has been reported that the stelar K+ outward rectifying channel (SKOR) is involved in loading K+ into the xylem for its transport from roots to shoots. Elytrigia elongata is a perennial, sparsely distributed, rhizome-type herbaceous plant belonging to the wheatgrass family; it has high salt tolerance. Here, we isolated EeSKOR from decaploid E. elongata and investigated its function in transgenic tobacco. The results showed that EeSKOR was mainly expressed in the roots and was up-regulated with increasing salinity and drought intensity. Overexpression of EeSKOR in plants exposed to salt stress enhanced growth performance, increased SOD activity and chlorophyll content, significantly reduced H2O2 and MDA content, reduced Na+ concentration, and increased K+ concentration in transgenic tobacco plants compared with wild-type (WT) and null vector (Vector) plants. Our findings suggest that transgenic plants overexpressing EeSKOR could enhance K+ transport from the roots to the aboveground parts to maintain K+ steady-state in the aboveground under conditions of salt stress, thereby enhancing tobacco salt tolerance.
Keywords
Elytrigia elongata, SKOR, Salt tolerance, Long-distance K+ transport
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Lin Meng
Beijing Academy of Agriculture and Forestry Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 14 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
As an important nutrient, K+ plays a crucial role in plant stress resistance. It has been reported that the stelar K+ outward rectifying channel (SKOR) is involved in loading K+ into the xylem for its transport from roots to shoots. Elytrigia elongata is a perennial, sparsely distributed, rhizome-type herbaceous plant belonging to the wheatgrass family; it has high salt tolerance. Here, we isolated EeSKOR from decaploid E. elongata and investigated its function in transgenic tobacco. The results showed that EeSKOR was mainly expressed in the roots and was up-regulated with increasing salinity and drought intensity. Overexpression of EeSKOR in plants exposed to salt stress enhanced growth performance, increased SOD activity and chlorophyll content, significantly reduced H2O2 and MDA content, reduced Na+ concentration, and increased K+ concentration in transgenic tobacco plants compared with wild-type (WT) and null vector (Vector) plants. Our findings suggest that transgenic plants overexpressing EeSKOR could enhance K+ transport from the roots to the aboveground parts to maintain K+ steady-state in the aboveground under conditions of salt stress, thereby enhancing tobacco salt tolerance.
Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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