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Research Article
Kirill Ukhanov
University of Florida
Corresponding Author
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Functional characterization of mammalian olfactory receptors (ORs) remains a major challenge to ultimately understanding the olfactory code. Here, we compare the responses of the mouse Olfr73 ectopically expressed in olfactory sensory neurons using AAV gene delivery in vivo and expressed in vitro in cell culture. The response dynamics and concentration-dependence of agonists for the ectopically expressed Olfr73 were similar to those reported for the endogenous Olfr73, but the receptor failed to mediate the antagonism between its cognate agonist and several antagonists reported for the OR expressed in vitro. Expressing the OR in vitro under the same conditions of stimulation retained the reported antagonism, but it was dependent on the duration of stimulation. Our findings suggest that both the cellular environment and the stimulus dynamics shape the functionality of Olfr73 and argue that characterizing ORs in ‘native’ conditions provides the most complete understanding of ligand-OR interactions.
Keywords
olfactory receptors (ORs), Olfr73, vivo, vitro
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View the published article at Scientific Reports.
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Posted 23 Dec, 2020
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Editorial decision: Major revision
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Kirill Ukhanov
University of Florida
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 23 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 18 Feb, 2021
Reviews received
Received 29 Dec, 2020
Reviewers agreed
On 25 Dec, 2020
Reviewers invited
Invitations sent on 22 Dec, 2020
Editor assigned
On 22 Dec, 2020
Editor invited
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
First submitted
On 07 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
Functional characterization of mammalian olfactory receptors (ORs) remains a major challenge to ultimately understanding the olfactory code. Here, we compare the responses of the mouse Olfr73 ectopically expressed in olfactory sensory neurons using AAV gene delivery in vivo and expressed in vitro in cell culture. The response dynamics and concentration-dependence of agonists for the ectopically expressed Olfr73 were similar to those reported for the endogenous Olfr73, but the receptor failed to mediate the antagonism between its cognate agonist and several antagonists reported for the OR expressed in vitro. Expressing the OR in vitro under the same conditions of stimulation retained the reported antagonism, but it was dependent on the duration of stimulation. Our findings suggest that both the cellular environment and the stimulus dynamics shape the functionality of Olfr73 and argue that characterizing ORs in ‘native’ conditions provides the most complete understanding of ligand-OR interactions.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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