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Research article
Expression of Epithelial-Mesenchymal Transition Related Markers in Plasmacytoid Urothelial Carcinoma of the Urinary Bladder
Shunichiro Nomura
Nippon Medical School
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4392-1278
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Urology.
Background: Plasmacytoid urothelial carcinoma (PUC) of the urinary bladder is a variant of urothelial carcinoma that carries a poor prognosis. The epithelial-mesenchymal transition (EMT) has been demonstrated to contribute to tumor progression. As the cause of the increased aggressiveness of PUC is unknown, we investigated PUC and EMT-related marker expression.
Methods: A total of 633 bladder carcinoma cases diagnosed from 2006 to 2015 at the Nippon Medical School Hospital were analyzed. Twelve patients were found to have plasmacytoid histology and diagnosed with PUC. Slides were evaluated for percentage of plasmacytoid variant, and stained for E-cadherin, N-cadherin, Vimentin, Fibronectin and Snail expression.
Results: The incidence of PUC was 1.9% (12/633). The median patient age at diagnosis was 71 years (range, 60–80 years) and the male-female ratio was 11:1. All but three patients had stage T2b or higher. The median overall survival was 10 months. In 10/12 cases, Snail and N-cadherin were positive. Vimentin was positive in 9/12 cases. Fibronectin was positive in 8/12 cases. While E-cadherin was negative in 10/12 cases. Nine cases showed >10% plasmacytoid component. Eight of the nine patients (88.9%) with >10% plasmacytoid component died.
Conclusions: The results indicate that PUC may induce EMT and may be associated with high invasion.
Keywords
bladder, urinary bladder, plasmacytoid, urothelial carcinoma, E-cadherin, Snail, epithelial-mesenchymal transition
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CURRENT STATUS: Published
Version 3
Posted 15 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 09 Jun, 2020
Review #2 received
Received 02 Jun, 2020
Review #1 received
Received 24 May, 2020
Reviewer #2 agreed
On 16 May, 2020
Reviewers invited
Invitations sent on 13 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Editor assigned
On 23 Apr, 2020
Submission checks complete
On 22 Apr, 2020
Editor invited
On 22 Apr, 2020
No comments provided
Editorial decision: Major revision
On 01 Apr, 2020
Review #3 received
Received 23 Mar, 2020
Reviewer #3 agreed
On 16 Mar, 2020
Review #2 received
Received 22 Feb, 2020
Review #1 received
Received 22 Feb, 2020
Reviewer #2 agreed
On 07 Feb, 2020
Reviewer #1 agreed
On 03 Feb, 2020
Editor assigned
On 01 Feb, 2020
Reviewers invited
Invitations sent on 01 Feb, 2020
Submission checks complete
On 24 Jan, 2020
Editor invited
On 24 Jan, 2020
First submitted
On 22 Jan, 2020
Metrics
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Subject Areas
Urology & Nephrology
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This preprint is under consideration at BMC Urology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Expression of Epithelial-Mesenchymal Transition Related Markers in Plasmacytoid Urothelial Carcinoma of the Urinary Bladder
Shunichiro Nomura
Nippon Medical School
Corresponding Author
ORCiD: https://orcid.org/0000-0002-4392-1278
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 15 Jun, 2020
No comments provided
Editorial decision: Minor revision
On 09 Jun, 2020
Review #2 received
Received 02 Jun, 2020
Review #1 received
Received 24 May, 2020
Reviewer #2 agreed
On 16 May, 2020
Reviewers invited
Invitations sent on 13 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Editor assigned
On 23 Apr, 2020
Submission checks complete
On 22 Apr, 2020
Editor invited
On 22 Apr, 2020
No comments provided
Editorial decision: Major revision
On 01 Apr, 2020
Review #3 received
Received 23 Mar, 2020
Reviewer #3 agreed
On 16 Mar, 2020
Review #2 received
Received 22 Feb, 2020
Review #1 received
Received 22 Feb, 2020
Reviewer #2 agreed
On 07 Feb, 2020
Reviewer #1 agreed
On 03 Feb, 2020
Editor assigned
On 01 Feb, 2020
Reviewers invited
Invitations sent on 01 Feb, 2020
Submission checks complete
On 24 Jan, 2020
Editor invited
On 24 Jan, 2020
First submitted
On 22 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Urology & Nephrology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Urology.
Background: Plasmacytoid urothelial carcinoma (PUC) of the urinary bladder is a variant of urothelial carcinoma that carries a poor prognosis. The epithelial-mesenchymal transition (EMT) has been demonstrated to contribute to tumor progression. As the cause of the increased aggressiveness of PUC is unknown, we investigated PUC and EMT-related marker expression.
Methods: A total of 633 bladder carcinoma cases diagnosed from 2006 to 2015 at the Nippon Medical School Hospital were analyzed. Twelve patients were found to have plasmacytoid histology and diagnosed with PUC. Slides were evaluated for percentage of plasmacytoid variant, and stained for E-cadherin, N-cadherin, Vimentin, Fibronectin and Snail expression.
Results: The incidence of PUC was 1.9% (12/633). The median patient age at diagnosis was 71 years (range, 60–80 years) and the male-female ratio was 11:1. All but three patients had stage T2b or higher. The median overall survival was 10 months. In 10/12 cases, Snail and N-cadherin were positive. Vimentin was positive in 9/12 cases. Fibronectin was positive in 8/12 cases. While E-cadherin was negative in 10/12 cases. Nine cases showed >10% plasmacytoid component. Eight of the nine patients (88.9%) with >10% plasmacytoid component died.
Conclusions: The results indicate that PUC may induce EMT and may be associated with high invasion.
Figure 1