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Research
Bamidele Tolulope Odumosu
University of Lagos
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8316-5174
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Background The presence of antibiotic resistant microorganisms in food is of great concern globally. This research was carried out to detect and characterize plasmid carriage and profiles among members of Enterobacteriaceae from different meat types in Nigeria.
Method From a total of 80 meat samples comprising of mutton, pork, beef and chicken, organisms belonging to the family Enterobacteriaceae were isolated by standard procedures and identified by API 20E system. Antibiotics susceptibilities testing (AST) against selected classes of antimicrobial agents and plasmid extraction was carried out by disc diffusion and alkaline lysis methods respectively.
Results One-hundred and ten Enterobacteriaceae were isolated, species identification revealed isolates belonging to 7 genera comprising of Escherichia, Enterobacter, Klebsiella, Citrobacter, Proteus, Salmonella and Serratia. Overall resistance of the organisms to amoxycillin/clavulanic acid was 91 (82.7%), streptomycin 85 (75.7%) and perfloxacin 74 (67.2%) while ofloxacin had the highest susceptibility rate (91.8%). Plasmids profiling revealed ranges of plasmids from 1 to 3 copies with estimated sizes range of 700bp to 1.1kb among E. coli, K. pneumoniae, E. aerogenes and Proteus mirabilis. All the isolates with plasmids were multidrug resistant and were isolated from chicken except a strain of E. coli from pork which harboured a single plasmid copy suggesting these meat as reservoirs for antibiotic resistant bacteria.
Conclusion Our findings revealed high level of meat contamination with antibiotic resistant Enterobacteriaceae harbouring resistant plasmids. An integrated surveillance system and safety practice must be ensured among the processors and retailers
Keywords
Enterobacteriaceae, plasmids, antibiotic, resistance
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CURRENT STATUS: Under Review
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Posted 16 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 15 Mar, 2021
Review #2 received
Received 08 Mar, 2021
Review #1 received
Received 06 Mar, 2021
Reviewer #2 agreed
On 03 Mar, 2021
Reviewer #1 agreed
On 16 Feb, 2021
Reviewers invited
Invitations sent on 28 Jan, 2021
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On 08 Dec, 2020
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On 08 Dec, 2020
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This preprint is under consideration at One Health Outlook. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Bamidele Tolulope Odumosu
University of Lagos
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8316-5174
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 15 Mar, 2021
Review #2 received
Received 08 Mar, 2021
Review #1 received
Received 06 Mar, 2021
Reviewer #2 agreed
On 03 Mar, 2021
Reviewer #1 agreed
On 16 Feb, 2021
Reviewers invited
Invitations sent on 28 Jan, 2021
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
First submitted
On 07 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background The presence of antibiotic resistant microorganisms in food is of great concern globally. This research was carried out to detect and characterize plasmid carriage and profiles among members of Enterobacteriaceae from different meat types in Nigeria.
Method From a total of 80 meat samples comprising of mutton, pork, beef and chicken, organisms belonging to the family Enterobacteriaceae were isolated by standard procedures and identified by API 20E system. Antibiotics susceptibilities testing (AST) against selected classes of antimicrobial agents and plasmid extraction was carried out by disc diffusion and alkaline lysis methods respectively.
Results One-hundred and ten Enterobacteriaceae were isolated, species identification revealed isolates belonging to 7 genera comprising of Escherichia, Enterobacter, Klebsiella, Citrobacter, Proteus, Salmonella and Serratia. Overall resistance of the organisms to amoxycillin/clavulanic acid was 91 (82.7%), streptomycin 85 (75.7%) and perfloxacin 74 (67.2%) while ofloxacin had the highest susceptibility rate (91.8%). Plasmids profiling revealed ranges of plasmids from 1 to 3 copies with estimated sizes range of 700bp to 1.1kb among E. coli, K. pneumoniae, E. aerogenes and Proteus mirabilis. All the isolates with plasmids were multidrug resistant and were isolated from chicken except a strain of E. coli from pork which harboured a single plasmid copy suggesting these meat as reservoirs for antibiotic resistant bacteria.
Conclusion Our findings revealed high level of meat contamination with antibiotic resistant Enterobacteriaceae harbouring resistant plasmids. An integrated surveillance system and safety practice must be ensured among the processors and retailers
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