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Research
Nana Yaw Sarpong
Kwame Nkrumah University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7099-2138
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Increasing global wood demands have necessitated an overall push for cost-effective methods of vegetative propagation using cuttings. Vegetative plant propagation through cuttings although a breakthrough is mostly hampered with the inability of cuttings to root effectively to achieve maximum yield. Ten clones each of Eucalyptus grandis x Eucalyptus urophylla (E. grandis x E. urophylla) and Eucalyptus pellita (E. pellita) were treated with Indole-3-butyric acid (IBA) concentrations of 0 ppm (control), 2000 ppm, 3000 ppm and 4000 ppm respectively.
Results
Overall mean survival of cuttings was low (< 50%) for all concentrations of IBA used in this research. Survival rates of individual clones however varied from clone to clone. E. grandis x E. urophylla cuttings had the highest mean survival (43.39%) when treated with 2000 ppm of IBA and lowest mean survival (37.86%) when treated with 0 ppm. Results from the survival rates of E. grandis × E. Urophylla and Eucalyptus pellita cuttings were subjected to an Analysis of Variance test to identify any significant differences between hormone concentration and cutting survival. ANOVA results indicated that differences between cutting–IBA concentrations for E. grandis × E. Urophylla were significant (p=1.85x10-08) which is lower than standard p value (p=0.05) used in the test.
For E. pellita (P) cuttings the highest mean survival (27.14%) was achieved when treated with 2000 ppm of Indole-3-butyric acid (IBA). Individual clones however showed significant differences in survival. E. pellita clone P2 achieved the highest survival (52.68%) in the control experiment (0 ppm). Some clones i.e P51 and P82 achieved survivals of less than 10% in all four levels of IBA
Conclusion
The best surviving Eucalyptus grandis x Eucalyptus urophylla clone (GH11) achieved a mean survival rate of 75% when the GH11 basal cutting was treated with 2000 ppm IBA. The results indicated that the production of clones with survival rates of less than 20% should be discarded since it makes no economic sense clones with survival rates of more than 40% should be planted out in the field to compare field performance with greenhouse survival.
Keywords
Clone, auxin, adventitious rooting, hybrid, cutting, ortet
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Nana Yaw Sarpong
Kwame Nkrumah University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7099-2138
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Tropical Agroecosystems.
Version 1
Posted 15 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Forestry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Tropical Agroecosystems.
Background
Increasing global wood demands have necessitated an overall push for cost-effective methods of vegetative propagation using cuttings. Vegetative plant propagation through cuttings although a breakthrough is mostly hampered with the inability of cuttings to root effectively to achieve maximum yield. Ten clones each of Eucalyptus grandis x Eucalyptus urophylla (E. grandis x E. urophylla) and Eucalyptus pellita (E. pellita) were treated with Indole-3-butyric acid (IBA) concentrations of 0 ppm (control), 2000 ppm, 3000 ppm and 4000 ppm respectively.
Results
Overall mean survival of cuttings was low (< 50%) for all concentrations of IBA used in this research. Survival rates of individual clones however varied from clone to clone. E. grandis x E. urophylla cuttings had the highest mean survival (43.39%) when treated with 2000 ppm of IBA and lowest mean survival (37.86%) when treated with 0 ppm. Results from the survival rates of E. grandis × E. Urophylla and Eucalyptus pellita cuttings were subjected to an Analysis of Variance test to identify any significant differences between hormone concentration and cutting survival. ANOVA results indicated that differences between cutting–IBA concentrations for E. grandis × E. Urophylla were significant (p=1.85x10-08) which is lower than standard p value (p=0.05) used in the test.
For E. pellita (P) cuttings the highest mean survival (27.14%) was achieved when treated with 2000 ppm of Indole-3-butyric acid (IBA). Individual clones however showed significant differences in survival. E. pellita clone P2 achieved the highest survival (52.68%) in the control experiment (0 ppm). Some clones i.e P51 and P82 achieved survivals of less than 10% in all four levels of IBA
Conclusion
The best surviving Eucalyptus grandis x Eucalyptus urophylla clone (GH11) achieved a mean survival rate of 75% when the GH11 basal cutting was treated with 2000 ppm IBA. The results indicated that the production of clones with survival rates of less than 20% should be discarded since it makes no economic sense clones with survival rates of more than 40% should be planted out in the field to compare field performance with greenhouse survival.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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