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Background: Lyme neuroborreliosis (LNB) is one of the most dangerous manifestations of Lyme disease, but the pathogenesis and inflammatory mechanisms are not fully understood.
Methods: Cultured explants from the frontal cortex of rhesus monkey brain (n=3) were treated with live Borrelia burgdorferi (Bb) or phosphate-buffered saline (PBS) for 6, 12, and 24 h. Total protein was collected for sequencing and bioinformatics analysis. Changes in protein expression in the explants over time following Bb infection were screened.
Results: We identified 1237 differentially expressed proteins (DEPs; fold change ≥1.5 or ≤0.67, P-value ≤0.05). One of these, growth-associated protein 43 (GAP-43), was highly expressed at all time points in the explants. The results of the protein-protein interaction network analysis of DEPs suggested that GAP-43 plays a role in the neuroinflammation associated with LNB. In HMC3 cells incubated with live Bb or PBS for 6, 12, and 24 h, real-time PCR and western blot analyses confirmed the upregulation of GAP-43 mRNA and protein, respectively.
Conclusions: Elevated GAP-43 expression is a potential marker for LNB that may be useful for diagnosis or treatment.
Keywords
Proteomic analysis, Lyme neuroborreliosis, Neuroinflammation, HMC3, Borrelia burgdorferi, GAP-43
Figure 1
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Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalTable1GOenrichmentofDEPs.xlsx
Table 1. GO enrichment of DEPs.
- SupplementalTable2PathwayenrichmentofDEPs.xlsx
Table 2. Pathway enrichment of DEPs.
- SupplementalTable3ScreeningofDEPs.xlsx
Table 3. Screening of DEPs.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Neurobiology of Disease
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Lyme neuroborreliosis (LNB) is one of the most dangerous manifestations of Lyme disease, but the pathogenesis and inflammatory mechanisms are not fully understood.
Methods: Cultured explants from the frontal cortex of rhesus monkey brain (n=3) were treated with live Borrelia burgdorferi (Bb) or phosphate-buffered saline (PBS) for 6, 12, and 24 h. Total protein was collected for sequencing and bioinformatics analysis. Changes in protein expression in the explants over time following Bb infection were screened.
Results: We identified 1237 differentially expressed proteins (DEPs; fold change ≥1.5 or ≤0.67, P-value ≤0.05). One of these, growth-associated protein 43 (GAP-43), was highly expressed at all time points in the explants. The results of the protein-protein interaction network analysis of DEPs suggested that GAP-43 plays a role in the neuroinflammation associated with LNB. In HMC3 cells incubated with live Bb or PBS for 6, 12, and 24 h, real-time PCR and western blot analyses confirmed the upregulation of GAP-43 mRNA and protein, respectively.
Conclusions: Elevated GAP-43 expression is a potential marker for LNB that may be useful for diagnosis or treatment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalTable1GOenrichmentofDEPs.xlsx
Table 1. GO enrichment of DEPs.
- SupplementalTable2PathwayenrichmentofDEPs.xlsx
Table 2. Pathway enrichment of DEPs.
- SupplementalTable3ScreeningofDEPs.xlsx
Table 3. Screening of DEPs.
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