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Development of an efficient tef-1α RNA hairpin structure to efficient management of lasiodiplodia theobromae
Abdolbaset Azizi
University of Kurdistan
Corresponding Author
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Lasiodiplodia theobromae is a serious worldwide-distributed plant pathogenic fungus with a wide host range in temperate and tropical climates. L. theobromae causes fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy to management of the diseases caused by L. theobromae. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on tef-1α gene to management of L. theobromae. Hairpin structure of tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to reduction in disease progress. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to management of L. theobromae for the first time.
Keywords
Botryosphaeriaceae, RNA silencing, Gene construct, Lasiodiplodia
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Development of an efficient tef-1α RNA hairpin structure to efficient management of lasiodiplodia theobromae
Abdolbaset Azizi
University of Kurdistan
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 18 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 12 Feb, 2021
Review #2 received
Received 09 Jan, 2021
Reviewer #3 agreed
On 05 Jan, 2021
Review #7 received
Received 04 Jan, 2021
Review #1 received
Received 04 Jan, 2021
Review #3 received
Received 04 Jan, 2021
Review #5 received
Received 04 Jan, 2021
Review #4 received
Received 04 Jan, 2021
Review #8 received
Received 04 Jan, 2021
Review #11 received
Received 04 Jan, 2021
Review #9 received
Received 04 Jan, 2021
Review #6 received
Received 04 Jan, 2021
Review #10 received
Received 04 Jan, 2021
Reviewer #8 agreed
On 04 Jan, 2021
Reviewer #2 agreed
On 04 Jan, 2021
Reviewer #4 agreed
On 04 Jan, 2021
Reviewer #6 agreed
On 04 Jan, 2021
Reviewer #5 agreed
On 04 Jan, 2021
Reviewer #9 agreed
On 04 Jan, 2021
Reviewer #1 agreed
On 04 Jan, 2021
Reviewer #12 agreed
On 04 Jan, 2021
Reviewer #10 agreed
On 04 Jan, 2021
Reviewer #7 agreed
On 04 Jan, 2021
Reviewer #11 agreed
On 04 Jan, 2021
Reviewers invited
Invitations sent on 04 Jan, 2021
Editor assigned
On 04 Jan, 2021
Editor invited
On 16 Dec, 2020
Submission checks complete
On 16 Dec, 2020
First submitted
On 11 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Lasiodiplodia theobromae is a serious worldwide-distributed plant pathogenic fungus with a wide host range in temperate and tropical climates. L. theobromae causes fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy to management of the diseases caused by L. theobromae. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on tef-1α gene to management of L. theobromae. Hairpin structure of tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to reduction in disease progress. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to management of L. theobromae for the first time.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5