Development of an efficient tef-1α RNA hairpin structure to efficient management of lasiodiplodia theobromae
Lasiodiplodia theobromae is a serious worldwide-distributed plant pathogenic fungus with a wide host range in temperate and tropical climates. L. theobromae causes fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy to management of the diseases caused by L. theobromae. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on tef-1α gene to management of L. theobromae. Hairpin structure of tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to reduction in disease progress. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to management of L. theobromae for the first time.
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Posted 18 Dec, 2020
On 12 Feb, 2021
Received 09 Jan, 2021
On 05 Jan, 2021
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On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
Invitations sent on 04 Jan, 2021
On 04 Jan, 2021
On 16 Dec, 2020
On 16 Dec, 2020
On 11 Dec, 2020
Development of an efficient tef-1α RNA hairpin structure to efficient management of lasiodiplodia theobromae
Posted 18 Dec, 2020
On 12 Feb, 2021
Received 09 Jan, 2021
On 05 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
Received 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
On 04 Jan, 2021
Invitations sent on 04 Jan, 2021
On 04 Jan, 2021
On 16 Dec, 2020
On 16 Dec, 2020
On 11 Dec, 2020
Lasiodiplodia theobromae is a serious worldwide-distributed plant pathogenic fungus with a wide host range in temperate and tropical climates. L. theobromae causes fruit rot, canker, and dieback of twigs in various woody plants. Protection of pruning wounds using fungicides is the prevalent strategy to management of the diseases caused by L. theobromae. Chemical control of plant diseases is not environmentally safe and the residues of fungicides are a threat to nature. Furthermore, genetic resources of resistance to plant diseases in woody plants are limited. The aim of this study was to investigate the efficiency of RNA silencing using an efficient hairpin structure based on tef-1α gene to management of L. theobromae. Hairpin structure of tef-1α was cloned in pFGC5941 binary vector and the recombinant construct was named pFGC-TEF-d. Transient expression of pFGC-TEF-d using Agrobacterium LBA4404 in grapevine (Bidaneh Sefid cv.) and strawberry cultivars (Camarosa and Ventana) led to reduction in disease progress. The disease reduction in grapevine was estimated by 55% and in strawberries cultivars Camarosa and Ventana by 58% and 93%, respectively. Here we introduce RNAi silencing using pFGC-TEF-d construct as an efficient strategy to management of L. theobromae for the first time.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5