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Research Article
Kok Song Lai
Higher Colleges of Technology
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Lignosulfonate (LS) is a by-product obtained during sulfite pulping process and is commonly used as a growth enhancer in plant growth. However, the underlying growth promoting mechanism of LS on shoot growth remains largely unknown. Hence, this study was undertaken to determine the potential application of eco-friendly LS chelated ion complex (NaLS and CaLS) to enhance recalcitrant indica rice MR219 shoot growth and to elucidate its underlying growth promoting mechanisms. The NaLS was shown to be a better shoot growth enhancer as compared to CaLS, with optimum concentration of 300 mg/L. Subsequent comparative proteomic analysis revealed an increase of photosynthesis-related proteins and stress regulator proteins abundance in NaLS-treated rice as compared to MSO (control). Consistently, biochemical analyses showed a significant increase of rubisco activity, total chlorophyll, total sugar and total protein contents in NaLS-treated rice, implying NaLS role in empowering photosynthesis activities that led to plant growth enhancement. In addition, low level of peroxidase activity, malondialdehyde content and phenylalanine ammonia lyase activity were also observed in NaLS-treated rice. These results suggest that NaLS plays a role in modulating cellular homeostasis to provide a conducive cellular environment for plant growth. Taken together, NaLS improved shoot growth of recalcitrant MR219 rice by upregulation of photosynthetic activities and reduction of cellular stress leading to better plant growth.
Keywords
Lignosulfonate, plant, environment
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CURRENT STATUS: Under Review
Version 1
Posted 23 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 03 Mar, 2021
Reviews received
Received 18 Feb, 2021
Reviewers agreed
On 02 Feb, 2021
Reviewers agreed
On 30 Jan, 2021
Reviewers invited
Invitations sent on 22 Jan, 2021
Editor assigned
On 21 Jan, 2021
Editor invited
On 21 Dec, 2020
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On 21 Dec, 2020
First submitted
On 11 Dec, 2020
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Kok Song Lai
Higher Colleges of Technology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 23 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 03 Mar, 2021
Reviews received
Received 18 Feb, 2021
Reviewers agreed
On 02 Feb, 2021
Reviewers agreed
On 30 Jan, 2021
Reviewers invited
Invitations sent on 22 Jan, 2021
Editor assigned
On 21 Jan, 2021
Editor invited
On 21 Dec, 2020
Submission checks complete
On 21 Dec, 2020
First submitted
On 11 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Lignosulfonate (LS) is a by-product obtained during sulfite pulping process and is commonly used as a growth enhancer in plant growth. However, the underlying growth promoting mechanism of LS on shoot growth remains largely unknown. Hence, this study was undertaken to determine the potential application of eco-friendly LS chelated ion complex (NaLS and CaLS) to enhance recalcitrant indica rice MR219 shoot growth and to elucidate its underlying growth promoting mechanisms. The NaLS was shown to be a better shoot growth enhancer as compared to CaLS, with optimum concentration of 300 mg/L. Subsequent comparative proteomic analysis revealed an increase of photosynthesis-related proteins and stress regulator proteins abundance in NaLS-treated rice as compared to MSO (control). Consistently, biochemical analyses showed a significant increase of rubisco activity, total chlorophyll, total sugar and total protein contents in NaLS-treated rice, implying NaLS role in empowering photosynthesis activities that led to plant growth enhancement. In addition, low level of peroxidase activity, malondialdehyde content and phenylalanine ammonia lyase activity were also observed in NaLS-treated rice. These results suggest that NaLS plays a role in modulating cellular homeostasis to provide a conducive cellular environment for plant growth. Taken together, NaLS improved shoot growth of recalcitrant MR219 rice by upregulation of photosynthetic activities and reduction of cellular stress leading to better plant growth.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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