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Research Article
Ibtissem Lahmar
Laboratoire de Parasitologie–Mycologie Médicale et Moléculaire (code LR12ES08), Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Tunisie
Corresponding Author
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The isolation and molecular typing of Toxoplasma gondii strains provides an essential basis for a better understanding of the distribution of genetic diversity and associated human health risks. In this context, we report, the molecular characterization of strains of T. gondii isolated from domestic animals infected with T. gondii in Southern and coastal area of Tunisia. This diversity is compared to that known for the Western European and Mediterranean sub-region.
Blood, hearts and/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples were performed using mouse bioassay. The strains genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers.
Viable strains of T. gondii were isolated from 13.4% sheep and 33.3% chickens. Furthermore, the parasite was also detected in three DNA extracts from animal tissue digestates.
This study showed a large predominance of type II strains (87.9%) from which two samples were type II variants for W35 locus. The other genotypes were three type III sheep isolates (9.1%) and, for the first time in Tunisia, an isolate of sheep origin of the Africa 4 genotype (3.0%). The comparison of microsatellite alleles of type II strains shows the recent migration of strains between Tunisia and other countries of the world.
Keywords
Toxoplasma gondii, Tunisia, sheep, free range chickens, microsatellite genotyping, Type II genotype
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This is a list of supplementary files associated with this preprint. Click to download.
- supplementarytables.pdf
Supplementary Table S1. Epidemiological data, serology results of 33 T. gondii positive samples isolated from sheep and free-range chickens of Tunisia Supplementary Table S2. Real Time PCR and genotyping results of T. gondii DNA with 15 microsatellite markers from 33 isolates collected from sheep and free-range chickens of Tunisia
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See the published version of this preprint at Scientific Reports.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Ibtissem Lahmar
Laboratoire de Parasitologie–Mycologie Médicale et Moléculaire (code LR12ES08), Département de Biologie Clinique B, Faculté de Pharmacie de Monastir, Université de Monastir, Tunisie
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Scientific Reports.
Version 1
Posted 31 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 01 Mar, 2021
Reviews received
Received 23 Feb, 2021
Reviewers agreed
On 08 Feb, 2021
Reviews received
Received 25 Jan, 2021
Reviewers agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 12 Jan, 2021
Editor assigned
On 07 Jan, 2021
Editor invited
On 28 Dec, 2020
Submission checks complete
On 28 Dec, 2020
First submitted
On 12 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Scientific Reports.
The isolation and molecular typing of Toxoplasma gondii strains provides an essential basis for a better understanding of the distribution of genetic diversity and associated human health risks. In this context, we report, the molecular characterization of strains of T. gondii isolated from domestic animals infected with T. gondii in Southern and coastal area of Tunisia. This diversity is compared to that known for the Western European and Mediterranean sub-region.
Blood, hearts and/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples were performed using mouse bioassay. The strains genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers.
Viable strains of T. gondii were isolated from 13.4% sheep and 33.3% chickens. Furthermore, the parasite was also detected in three DNA extracts from animal tissue digestates.
This study showed a large predominance of type II strains (87.9%) from which two samples were type II variants for W35 locus. The other genotypes were three type III sheep isolates (9.1%) and, for the first time in Tunisia, an isolate of sheep origin of the Africa 4 genotype (3.0%). The comparison of microsatellite alleles of type II strains shows the recent migration of strains between Tunisia and other countries of the world.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- supplementarytables.pdf
Supplementary Table S1. Epidemiological data, serology results of 33 T. gondii positive samples isolated from sheep and free-range chickens of Tunisia Supplementary Table S2. Real Time PCR and genotyping results of T. gondii DNA with 15 microsatellite markers from 33 isolates collected from sheep and free-range chickens of Tunisia
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