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Research Article
Hengguang Zhao
First Affiliated Hospital of Chongqing Medical University
Corresponding Author
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Psoriasis vulgaris is a chronic inflammatory skin disorder. Its pathogenesis is now still unelucidated and the treatment is far from satisfied. DNA Damage-Inducible Transcript 4 (DDIT4) is a widely expressed protein in different tissue, which can activate cell macro-autophagy through mTORC1 passway. Vitamin D3 and analogues is a classic topical reagent for psoriasis vulgaris for more than 30 years, but its exact mechanism is not fully clear. In this study, we intend to verify whether vitamin D3 also exerts anti-psoriasis through promoting DDIT4 inducing macro-autophagy and proliferation inhibition in psoriasis vulgaris. Results showed DDIT4 was over-expressed in psoriatic tissue, and probably acted as an innate protector during psoriasis pathogenesis. 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) could promote DDIT4 expression in a rough linear correlation and subsequently activate macro-autophagy and inhabit cell proliferation, especially at the high concentration of 100nM. In terms of our understanding, this is the first time to reveal the interactions between 1,25(OH)2D3, DDIT4 and macro-autophagy in psoriasis vulgaris. DDIT4 is also probably a potential therapeutic target in future psoriatic treatments.
Keywords
DNA Damage-Inducible Transcript 4, Psoriasis, 1,25 dihydroxyvitamin D3, Macro-autophagy, proliferation
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Hengguang Zhao
First Affiliated Hospital of Chongqing Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Psoriasis vulgaris is a chronic inflammatory skin disorder. Its pathogenesis is now still unelucidated and the treatment is far from satisfied. DNA Damage-Inducible Transcript 4 (DDIT4) is a widely expressed protein in different tissue, which can activate cell macro-autophagy through mTORC1 passway. Vitamin D3 and analogues is a classic topical reagent for psoriasis vulgaris for more than 30 years, but its exact mechanism is not fully clear. In this study, we intend to verify whether vitamin D3 also exerts anti-psoriasis through promoting DDIT4 inducing macro-autophagy and proliferation inhibition in psoriasis vulgaris. Results showed DDIT4 was over-expressed in psoriatic tissue, and probably acted as an innate protector during psoriasis pathogenesis. 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) could promote DDIT4 expression in a rough linear correlation and subsequently activate macro-autophagy and inhabit cell proliferation, especially at the high concentration of 100nM. In terms of our understanding, this is the first time to reveal the interactions between 1,25(OH)2D3, DDIT4 and macro-autophagy in psoriasis vulgaris. DDIT4 is also probably a potential therapeutic target in future psoriatic treatments.
Figure 1
Figure 2
Figure 3
Figure 4
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