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Research Article
Sun Young Kim
Asan Medical Center
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
For the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets were emerged. However, issues for the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small sized antibody fragment conjugated with FITC for the real-time detection of colorectal cancer by in vivo molecular endoscopy imaging. A small-sized scFv fragment targets colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissue, and its full-length IgG probe has subjected to study for molecular imaging previously. To assess the advanced efficacy of anti-CCSP-2 scFv-FITC, surgical specimens were obtained from 21 patients with colorectal cancer for ex vivo molecular fluorescence analysis, histology, and immunohistochemistry. Orthotopic mice were administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions were observed by real-time fluorescence colonoscopy. The fluorescence imaging of human colon cancer specimens allowed the distinction of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression level was found to be correlated with the fluorescence intensity. Here, we demonstrated the feasibility and safety of anti-CCSP-2 scFv-FITC for molecular imaging as well as its potential in real-time fluorescence colonoscopy for the differential diagnosis of tumor lesions.
Keywords
Colorectal cancer, Molecular imaging, CCSP-2, scFv antibody
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CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 19 Apr, 2021
Reviews received
Received 20 Mar, 2021
Reviewers agreed
On 17 Mar, 2021
Reviewers agreed
On 08 Mar, 2021
Reviewers invited
Invitations sent on 04 Jan, 2021
Editor assigned
On 04 Jan, 2021
Editor invited
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
First submitted
On 14 Dec, 2020
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A new preprint design is coming!
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Sun Young Kim
Asan Medical Center
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 19 Apr, 2021
Reviews received
Received 20 Mar, 2021
Reviewers agreed
On 17 Mar, 2021
Reviewers agreed
On 08 Mar, 2021
Reviewers invited
Invitations sent on 04 Jan, 2021
Editor assigned
On 04 Jan, 2021
Editor invited
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
First submitted
On 14 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
For the sensitive diagnosis of colorectal cancer lesions, advanced molecular imaging techniques using cancer-specific targets were emerged. However, issues for the clearance of unbound probes and immunogenicity remain unresolved. To overcome these limitations, we developed a small sized antibody fragment conjugated with FITC for the real-time detection of colorectal cancer by in vivo molecular endoscopy imaging. A small-sized scFv fragment targets colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissue, and its full-length IgG probe has subjected to study for molecular imaging previously. To assess the advanced efficacy of anti-CCSP-2 scFv-FITC, surgical specimens were obtained from 21 patients with colorectal cancer for ex vivo molecular fluorescence analysis, histology, and immunohistochemistry. Orthotopic mice were administered with anti-CCSP-2 scFv-FITC topically and intravenously, and distinct tumor lesions were observed by real-time fluorescence colonoscopy. The fluorescence imaging of human colon cancer specimens allowed the distinction of malignant tissues from non-malignant tissues (p < 0.05), and the CCSP-2 expression level was found to be correlated with the fluorescence intensity. Here, we demonstrated the feasibility and safety of anti-CCSP-2 scFv-FITC for molecular imaging as well as its potential in real-time fluorescence colonoscopy for the differential diagnosis of tumor lesions.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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