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Research Article
Sniffer Worm, C. elegans, as a Toxicity Evaluation Model Organism with Sensing and Locomotion Abilities
Haeshin Lee
Korea Advanced Institute of Science and Technology
Corresponding Author
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The probability of objects fabricated by three-dimensional (3D) printing exhibiting local defects is higher than that detected in products of conventional casting-based manufacturing. Multistep layer-by-layer procedures in additive manufacturing are the main reason. Light intensity and/or penetration depth, inhomogeneity of components, and variations in nozzle temperature are factors that create local defects. Defect regions are sources of toxic component release, but methods to identify them in printed materials have not been reported. Existing assays for evaluating material toxicity are based on extraction, and these toxicological assays use living creatures to passively detect harmful agents in extracted solutions. Thus, the development of an active system for identifying sites of toxicity sources is a critical and urgent issue in 3D printing technologies. Herein, we introduce an animal model system, C. elegans, for toxicity evaluation. C. elegans crawls toward safe regions but avoids toxically dangerous areas. The ‘sensing’ and ‘locomotion’ abilities of C. elegans are unparalleled among existing underwater and animal models, providing immediate indications to help find toxicity source sites.
Keywords
probability, three-dimensional (3D) printing, nozzle temperature, toxic component, animal model system
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Sniffer Worm, C. elegans, as a Toxicity Evaluation Model Organism with Sensing and Locomotion Abilities
Haeshin Lee
Korea Advanced Institute of Science and Technology
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
No community comments so far
Review #1 received
Received 12 Jan, 2021
Reviewer #2 agreed
On 01 Jan, 2021
Reviewer #4 agreed
On 01 Jan, 2021
Reviewer #3 agreed
On 01 Jan, 2021
Reviewer #1 agreed
On 01 Jan, 2021
Reviewer #5 agreed
On 01 Jan, 2021
Reviewer #6 agreed
On 01 Jan, 2021
Reviewers invited
Invitations sent on 29 Dec, 2020
Editor assigned
On 29 Dec, 2020
Editor invited
On 23 Dec, 2020
Submission checks complete
On 23 Dec, 2020
First submitted
On 14 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The probability of objects fabricated by three-dimensional (3D) printing exhibiting local defects is higher than that detected in products of conventional casting-based manufacturing. Multistep layer-by-layer procedures in additive manufacturing are the main reason. Light intensity and/or penetration depth, inhomogeneity of components, and variations in nozzle temperature are factors that create local defects. Defect regions are sources of toxic component release, but methods to identify them in printed materials have not been reported. Existing assays for evaluating material toxicity are based on extraction, and these toxicological assays use living creatures to passively detect harmful agents in extracted solutions. Thus, the development of an active system for identifying sites of toxicity sources is a critical and urgent issue in 3D printing technologies. Herein, we introduce an animal model system, C. elegans, for toxicity evaluation. C. elegans crawls toward safe regions but avoids toxically dangerous areas. The ‘sensing’ and ‘locomotion’ abilities of C. elegans are unparalleled among existing underwater and animal models, providing immediate indications to help find toxicity source sites.
Figure 1
Figure 2
Figure 3
Figure 4