DOI: https://doi.org/10.21203/rs.3.rs-1282126/v1
Background: The literature on the association between the atherogenic index of plasma (AIP) with the risk of major adverse cardiovascular events (MACEs) among non-diabetic hypertensive elderly patients is quite limited at present.
Methods: We performed a post-hoc analysis of data obtained from the Systolic Blood Pressure Intervention Trial (SPRINT) and explored the predictive value of AIP on the risk of MACEs among non-diabetic hypertensive elderly patients, to evaluate whether the benefit of intensive blood pressure control in preventing MACEs is stable in different AIP subgroups.
Results: 9,323 participants with AIP were included in this analysis, and 561(6.02%) participants had composite cardiovascular outcomes during a median of 3.22 years of follow-up. The subjects in the highest quartile of AIP had a significantly increased risk of the primary outcome. In the fully adjusted Model 3, the adjusted HRs of the primary outcome for participants in Q2, Q3, and Q4 of AIP were 1.32 (1.02, 1.72), 1.38 (1.05, 1.81), and 1.56 (1.17, 2.08) respectively. Consistently, the trend test for the association between AIP quartiles and the primary outcome showed that a higher AIP quartile was associated with a significantly higher risk of the primary outcome (adjusted HR (95%CI) in Model 3: 1.14 (1.04, 1.25), P=0,004). However, within each AIP quartile, absolute event rates were lower for the intensive treatment group. No evidence was found for the interaction of intensive blood pressure control and AIP for the risk of the primary outcome (P for interaction = 0.932).
Conclusion: This study found that elevated AIP is independently and positively associated with the risk of MACEs among non-diabetic elderly hypertensive patients. The benefit of intensive blood pressure control in managing cardiovascular events was consistent in different AIP subgroups.
Dyslipidemia is one of the known risk factors for cardiovascular diseases, which is the main cause of morbidity and mortality worldwide. The atherogenic index of plasma (AIP), calculated by the logarithm of the ratio between the level of triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C), is an indicator reflecting the characteristics and the degree of abnormal lipid metabolism.1 Previous studies revealed that AIP was associated with the risks of several cardiovascular diseases, such as incident ischemic heart disease, atherosclerosis, coronary artery disease, acute ischemic stroke, etc.2-4 However, previous studies have been mainly focusing on the general population. To our knowledge, information on the association between AIP with the risk of major adverse cardiovascular events (MACEs) among non-diabetic hypertensive elderly patients is very limited.
Compared with standard blood pressure control (<140/90 mm Hg), intensive blood pressure control (<120/80 mm Hg) has shown significantly better cardiovascular outcomes according to the results from the Systolic Blood Pressure Intervention Trial (SPRINT).5 It is less known whether the association between AIP and MACEs is independent of standard or intensive blood pressure control. Therefore, in this study, we aim to explore the predictive value of AIP on the risk of MACEs among non-diabetic hypertensive elderly patients and to evaluate whether the benefit of intensive blood pressure control in preventing MACEs is stable in different AIP subgroups.
Data used in this analysis was derived from the SPRINT dataset, available at National, Heart, Lung, and Blood Institute data repository in the Biologic Specimen and Data Repository Information Coordinating Center (BioLINCC). SPRINT was a randomized, controlled, open-label trial conducted at 102 clinical sites in the United States and approved by all institutional review boards involved in the participating centers. The protocol and main outcomes have previously been published.5,6 The primary outcome was that in patients with hypertension at high cardiovascular risk, intensive BP control could significantly reduce the rate of the composite cardiovascular outcomes and all-cause mortality compared with standard BP control.
The main aim of this analysis is to assess the association between baseline atherogenic index of plasma (calculated by triglycerides and HDL cholesterol) and the composite cardiovascular outcomes in the SPRINT participants. We excluded 38 subjects with missing baseline triglycerides and HDL cholesterol and 9,323 participants were included in the final analysis.
The atherogenic index of plasma was calculated by the logarithm of TG/HDL-C mole ratio base 10. The adopted conversion factors were TG: 1 ng/dL = 0.011 mmol/L, HDL-C: 1 ng/dL = 0.026 mmol/L. 9,323 participants were grouped according to AIP quartiles: Q1 (n=2331), Q2 (n=2329), Q3 (n=2330), Q4 (n=2333). The Q1 group was used as the reference.
The primary outcome of this analysis was the composite cardiovascular outcomes, including myocardial infarction (MI), no-MI acute coronary syndrome, stroke, cardiovascular mortality, and heart failure. The definition of the outcomes has been published in the SPRINT protocol.6
Participants were grouped by the AIP quartiles. Continuous variables were expressed as mean (standard deviation) or median (Q1-Q3) based on the distribution of data. The difference between the quartiles were tested using ANOVA and Kruskal-Wallis H test for normal distribution data and skew distribution data respectively. All categorical variables were expressed as frequency (percentile). Chi-square test or Fisher test were used to test the difference of categorical variables between groups.
We performed the Cox model to assess the association between AIP quartiles and the occurrence of the primary outcome in three models. The model was adjusted for none. Model 2 was adjusted for age, race, and treatment arms. Model 3 was adjusted for age, race, treatment arms, body mass index, systolic blood pressure, heart rate, smoking status, serum creatinine, fasting total cholesterol, fasting glucose, previous CVD, previous CKD, aspirin use, and statin use. The Schoenfeld residuals test was used to test the proportional hazard assumption in the Cox model. The robustness of the association between AIP and primary outcome was evaluated in prescribed subgroups using subgroup analysis and interaction test. To establish the relationship between AIP and the effect of intensive BP control for the risk of the primary outcome, P for interaction was calculated. The model was adjusted for all covariates in Model 3 except treatment arms. Poisson regression was used to assess the incidence of primary outcomes by treatment arms and AIP quartiles.
All analyses were performed using the statistical software packages R (The R Foundation; http://www.R-project.org). Statistical significance was set at P<0.05.
9,323 participants with AIP were included in this analysis, and 561(6.02%) participants had composite cardiovascular outcomes during a median of 3.22 years of follow-up. The baseline characteristics of the included subjects according to the AIP quartiles were shown in Table 1. Participants in higher quartiles of AIP had a higher body mass index, diastolic blood pressure, heart rate, serum creatinine, fasting triglycerides, glucose, and cardiovascular risk than in lower quartiles. Subjects in higher quartiles were more likely to be <75 years of age, male, and current smokers. Compared with participants in lower quartiles, subjects in higher quartiles were more likely to have chronic kidney disease and a higher rate of composite cardiovascular outcomes.
Table 2 presented the HRs (95%CI) of primary outcome among the included participants grouped by quartiles of AIP. The subjects in the highest quartile of AIP had a significantly increased risk of the primary outcome. The association persisted even after adjusting for potential confounding factors, including age, race, treatment arms, body mass index, systolic blood pressure, heart rate, smoking status, serum creatinine, fasting total cholesterol, fasting glucose, previous CVD, previous CKD, aspirin use and statin use. In the fully adjusted Model 3, the adjusted HRs of the primary outcome for participants in Q2, Q3, and Q4 of AIP were 1.32 (1.02, 1.72), 1.38 (1.05, 1.81), and 1.56 (1.17, 2.08) respectively. Consistently, the trend test for the association between AIP quartiles and the primary outcome showed that a higher AIP quartile was associated with a significantly higher risk of the primary outcome (adjusted HR (95%CI) in Model 3: 1.14 (1.04, 1.25), P=0,004).
The stratified analyses were performed to assess the impact of AIP (per 1 quartile increment) on the risk of the primary outcome. As shown in Table 3, the relationship between AIP and the primary outcome was consistent in prescribed stratifications: sex (male vs. female), age (<75 years vs. >=75 years), race (black vs. non-black), previous CVD (yes vs. no), previous CKD (yes vs. no), Framingham 10-y CVD risk (<15% vs. >=15%), aspirin use (yes vs. no) and statin use (yes vs. no). All P for interaction was>0.05.
Results stratified by AIP quartiles showed higher event rates with increasing frailty in both treatment groups (Figure 1). However, within each AIP quartile, absolute event rates were lower for the intensive treatment group. No evidence was found for the interaction of intensive blood pressure control and AIP for the risk of the primary outcome (P for interaction = 0.932).
This secondary analysis of the SPRINT data found that among hypertensive elderly patients, higher AIP was associated with a significantly higher risk of composite cardiovascular outcomes. The benefit of intensive blood pressure control on composite cardiovascular outcomes was consistent in different AIP quartiles. No evidence was found for the interaction of intensive blood pressure control and AIP for the risk of the primary outcome.
Previous studies observed a positive association between AIP and the risk of MACEs in different populations. A fifteen-year cohort study on healthy adults revealed the value of AIP in the prediction of developing cardiovascular events and its-related mortality.7 Results of a study on the Iran population indicated AIP to be positively associated with the risk of cardiovascular diseases.8 A cohort study among postmenopausal Chinese women (aged over 50) concluded that AIP was an independent predictor of cardiovascular risk. A study on the Health Risk Assessment Study and Korea Health Insurance Review and Assessment Service (HERAS-HIRA) cohort found that the higher the AIP quartile, the higher the incidence of ischemic heart disease.2 In the elderly population, those with a higher baseline blood pressure have a higher Framingham score, which represents a higher cardiovascular risk. Although a large body of evidence has proven the positive association between AIP and the risk of MACEs, this association was not fully discovered and needs to be studied in elderly patients with hypertension. Consistent with previous studies, in this study, we found elevated AIP to be positively associated with a higher risk of MACEs in non-diabetic elderly patients with hypertension.
TG level was suggested to be positively associated with a higher prevalence of cardiovascular outcomes.9 Within the high TG level, low HDL-C levels showed a higher risk of cardiovascular disease compared to people with low HDL-C levels.10 Increases in TG levels and decreases in HDL levels are both strong indicators of cardiovascular risk. This mechanism may explain why elevated AIP as a parameter of blood lipid levels has a strong correlation with increased risk of MACEs. In addition, a previous study found AIP level was correlated with the degree of insulin resistance, which indicated the degree of abnormal glucose metabolism.11 Insulin resistance and related hyperinsulinemia, hyperglycemia, as well as adipocytokines, may also cause vascular endothelial dysfunction, dyslipidemia, hypertension, and vascular inflammation, all of which contribute to the development of cardiovascular diseases.
According to a 9-year longitudinal study in Taiwan, AIP was proven to be significantly positively correlated with the prevalence of hypertension.12 A longitudinal study following a cohort of Hanzhong hypertensive adolescents for 12 years found that a high AIP level was a strong risk factor of hypertension-associated renal damage.13 With a growing body of evidence showing a linear relationship between blood pressure and the risk of cardiovascular diseases, the benefits of intensive blood pressure control in decreasing MACEs risks have already been proven in older patients with hypertension.14,15 Therefore, we evaluated the relationship between AIP and the effect of intensive blood pressure control for the risk of cardiovascular diseases and found the benefit of intensive blood pressure control is consistent in different AIP quartiles and no interaction of intensive blood pressure control and AIP for the risk of primary outcome was found.
Some limitations in this study should be addressed. First, the SPRINT trial did not define AIP subgroups, which limited the expansion of the results. Second, some AIP-related data, such as waist circumference and diet, cannot be acquired, while these data may alter the study results. Furthermore, an optimal cut-off value of AIP in predicting MACE risk remains to be determined for better risk stratification in clinical settings. Therefore, future investigations are necessary to evaluate the clinical application of AIP in non-diabetic elderly patients with hypertension.
In conclusion, this study found that elevated AIP is independently and positively associated with the risk of MACEs among non-diabetic elderly hypertensive patients. The benefit of intensive blood pressure control in managing cardiovascular events was consistent in different AIP subgroups. There is no need to pay too much attention to AIP when treating elderly hypertensive patients with intensive blood pressure regimen in clinical settings.
AIP: atherogenic index of plasma
MACEs: major adverse cardiovascular events
SPRINT: Systolic Blood Pressure Intervention Trial
TG: triglyceride
HDL-C: triglyceride and high-density lipoprotein cholesterol
MI: myocardial infarction
HERAS-HIRA: Health Risk Assessment Study and Korea Health Insurance Review and Assessment Service
Ethics approval and consent to participate
Not applicable.
Consent for publication
Not applicable.
Availability of data and materials
The dataset supporting the conclusions of this article is available in the Biologic Specimen and Data Repository Information Coordinating Center (BioLINCC) repository, hyperlink to dataset in https://biolincc.nhlbi.nih.gov/home/.
Competing interests
The authors declare that they have no competing interests.
Funding
This study was supported by the Fundamental Research Funds for Central Universities of Central South University (unique identifier: 2020zzts876).
Author’s contributions
FH and JC completed the writing of the paper. KZ applied for the database and made statistical analysis. ZW and YW were responsible for the revision of the paper. All authors confirmed the final version of the paper.
Acknowledgements
Not applicable.
Table 1. Baseline characteristic of the participants included in the analysis according to AIP quartiles.
Variable |
AIP |
P value |
|||
Q1 |
Q2 |
Q3 |
Q4 |
||
N |
2331 |
2329 |
2330 |
2333 |
|
AIP, mean (SD) |
-0.39 (0.12) |
-0.13 (0.05) |
0.05 (0.06) |
0.35 (0.17) |
<0.001 |
Female, n (%) |
1056 (45.30%) |
891 (38.26%) |
760 (32.62%) |
600 (25.72%) |
<0.001 |
Treatment |
|
|
|
|
|
Intensive, n (%) |
1168 (50.11%) |
1173 (50.36%) |
1171 (50.26%) |
1150 (49.29%) |
0.882 |
BMI(Kg/m2), median (Q1-Q3) |
28.03 (5.72) |
29.62 (5.76) |
30.59 (5.89) |
31.16 (5.18) |
|
Age, y |
|
|
|
|
|
Overall |
70.01 (9.53) |
68.44 (9.41) |
67.66 (9.19) |
65.51 (8.98) |
<0.001 |
≥75y, n (%) |
871 (37.37%) |
700 (30.06%) |
615 (26.39%) |
455 (19.50%) |
<0.001 |
Race, n (%) |
|
|
|
|
<0.001 |
Non-Hispanic White |
1207 (51.78%) |
1303 (55.95%) |
1382 (59.31%) |
1494 (64.04%) |
|
Non-Hispanic Black |
919 (39.43%) |
790 (33.92%) |
622 (26.70%) |
454 (19.46%) |
|
Hispanic |
160 (6.86%) |
201 (8.63%) |
280 (12.02%) |
337 (14.44%) |
|
Other |
45 (1.93%) |
35 (1.50%) |
46 (1.97%) |
48 (2.06%) |
|
Baseline blood pressure, mm Hg |
|
|
|
|
|
Systolic (mm Hg) |
140.94 (16.03) |
139.82 (15.52) |
139.20 (15.58) |
138.71 (15.09) |
<0.001 |
Diastolic (mm Hg) |
77.15 (12.42) |
78.00 (11.83) |
77.85 (11.45) |
79.51 (11.93) |
<0.001 |
Distribution of systolic blood pressure, n (%) |
|
|
<0.001 |
||
≤132 mm Hg |
712 (30.54%) |
792 (34.01%) |
798 (34.25%) |
822 (35.23%) |
|
>132 to <145 mm Hg |
738 (31.66%) |
738 (31.69%) |
771 (33.09%) |
779 (33.39%) |
|
≥145 mm Hg |
881 (37.79%) |
799 (34.31%) |
761 (32.66%) |
732 (31.38%) |
|
Baseline heart rate, bpm |
65.39 (11.33) |
65.79 (11.44) |
66.11 (11.72) |
67.61 (11.82) |
|
Serum creatinine, mg/dL |
1.04 (0.32) |
1.07 (0.32) |
1.07 (0.34) |
1.12 (0.37) |
<0.001 |
Urine Albumin/Creatinine ratio, mg/g Cr, median (Q1-Q3) |
9.84 (5.86-20.75) |
9.09 (5.49-19.97) |
9.33 (5.62-22.82) |
9.71 (5.57-23.00) |
0.047 |
Estimated GFR, mL.min−1 1.73 m−2, median (Q1-Q3) |
72.32 (59.75-86.22) |
70.92 (57.90-83.91) |
71.84 (58.38-84.95) |
70.17 (56.49-83.44) |
<0.001 |
Fasting total cholesterol, mg/dL, median (Q1-Q3) |
187.00 (162.00-214.00) |
183.00 (158.00-209.00) |
185.00 (158.00-212.00) |
193.00 (168.00-223.00) |
<0.001 |
Fasting total triglycerides, mg/dL, median (Q1-Q3) |
63.00 (54.00-74.00) |
90.00 (80.00-104.00) |
123.00 (109.00-138.00) |
194.00 (162.00-242.00) |
<0.001 |
Fasting HDL cholesterol, mg/dL, median (Q1-Q3) |
66.00 (57.00-76.00) |
53.00 (48.00-60.00) |
47.00 (42.00-53.00) |
40.00 (36.00-46.00) |
<0.001 |
Fasting glucose, mg/dL, median (Q1-Q3) |
95.00 (89.00-101.00) |
96.00 (90.00-104.00) |
98.00 (91.00-106.00) |
100.00 (93.00-109.00) |
<0.001 |
Statin use, n (%) |
916 (39.65%) |
1042 (44.99%) |
1081 (46.76%) |
1007 (43.33%) |
<0.001 |
Aspirin use, n (%) |
1182 (50.86%) |
1197 (51.42%) |
1224 (52.65%) |
1144 (49.16%) |
0.120 |
Smoking status, n (%) |
|
|
|
|
0.006 |
Never smoked |
1088 (46.68%) |
1031 (44.27%) |
1024 (43.95%) |
968 (41.49%) |
|
Former smoker |
964 (41.36%) |
994 (42.68%) |
1006 (43.18%) |
999 (42.82%) |
|
Current smoker |
276 (11.84%) |
302 (12.97%) |
296 (12.70%) |
364 (15.60%) |
|
Framingham 10-y CVD risk score, %, median (Q1-Q3) |
14.71 (10.27-21.61) |
16.80 (11.33-24.24) |
18.49 (12.68-26.77) |
21.39 (15.08-30.26) |
<0.001 |
Previous CVD, n (%) |
420 (18.02%) |
466 (20.01%) |
502 (21.55%) |
481 (20.62%) |
0.021 |
Previous CKD, n (%) |
596 (25.57%) |
673 (28.90%) |
641 (27.51%) |
735 (31.50%) |
<0.001 |
Primary outcome |
103 (4.42%) |
137 (5.88%) |
150 (6.44%) |
171 (7.33%) |
<0.001 |
AIP: atherogenic index of plasma.
Table 2. Association between AIP and primary outcome in different models.
AIP quartiles |
Model 1 |
Model 2 |
Model 3 |
HR (95%CI) P value |
|||
1 |
Ref. |
Ref. |
Ref. |
2 |
1.33 (1.03, 1.72) P=0.027 |
1.46 (1.13, 1.89) P=0.004 |
1.32 (1.02, 1.72) P=0.037 |
3 |
1.45 (1.13, 1.86) P=0.004 |
1.68 (1.31, 2.17) P<0.001 |
1.38 (1.05, 1.81) P=0.020 |
4 |
1.65 (1.29, 2.10) P<0.001 |
2.16 (1.68, 2.78) P<0.001 |
1.56 (1.17, 2.08) P=0.003 |
P for trend (1 Q increment) |
1.16 (1.08, 1.25) P<0.001 |
1.27 (1.18, 1.38) P<0.001 |
1.14 (1.04, 1.25) P=0.004 |
Model were adjusted for none. Model 2 were adjusted for age, race and treatment arms. Model 3 were adjusted for age, race, treatment arms, body mass index, systolic blood pressure, heart rate, smoking status, serum creatinine, fasting total cholesterol, fasting glucose, previous CVD, previous CKD, aspirin use and statin use.
Table 3. Subgroup analysis for the risk of primary outcome by baseline AIP quartiles (1 quartile increment).
|
AIP Quartiles (1 Q increment) |
|
|
Subgroup |
HR, 95%CI |
P value |
P for interaction |
Sex |
|
|
0.336 |
Male |
1.67 (1.11, 2.52) |
0.014 |
|
Female |
1.28 (0.80, 2.05) |
0.305 |
|
Age group |
|
|
0.816 |
<75 |
1.31 (0.89, 1.92) |
0.174 |
|
>=75 |
1.39 (0.91, 2.11) |
0.129 |
|
Race |
|
|
0.398 |
Black |
1.34 (0.77, 2.31) |
0.302 |
|
No-black |
1.72 (1.20, 2.47) |
|
|
Previous CVD |
|
|
0.932 |
Yes |
1.62 (1.01, 2.58) |
0.044 |
|
No |
1.65 (1.14, 2.40) |
0.008 |
|
Previous CkD |
|
|
0.981 |
Yes |
1.65 (1.04, 2.61) |
0.034 |
|
No |
1.64 (1.13, 2.38) |
0.01 |
|
CVD RISK |
|
|
0.423 |
<15% |
1.47 (0.88, 2.46) |
0.141 |
|
>=15% |
1.86 (1.27, 2.72) |
0.001 |
|
Aspirin Use |
|
|
0.912 |
Yes |
1.66 (1.13, 2.43) |
0.009 |
|
No |
1.61 (1.03, 2.52) |
0.035 |
|
Statin Use |
|
|
0.350 |
Yes |
1.84 (1.22, 2.77) |
0.003 |
|
No |
1.45 (0.96, 2.19) |
0.074 |
|
Model were adjusted for all covariates in Model 3 except stratifications itself.