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Research
Liang Qiu
Jiangxi University of Traditional Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5850-1455
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This work is licensed under a CC BY 4.0 License. Read Full License
Background
The gut microbiota plays important roles in the development of cardiovascular diseases. However, whether myocardial ischemia–reperfusion (I/R) alters gut microbiota composition and its significance remains unknown. In this work, Sprague–Dawley rats were subjected to left anterior descending coronary artery ligation and reperfusion. The fecal DNA isolated before myocardial ischemia and after reperfusion were analyzed for microbiota changes by high-throughput 16S rDNA sequencing.
Results
Results showed that compared with the pre-I/R and pre-sham surgery group, I/R and sham surgery significantly increased the relative abundance of the phyla Proteobacteria , and decreased the relative abundance of the phylum Firmicutes . The I/R surgical procedure increased the proportions of phylum Spirochaetes and genus Enterococcus compared to pre-I/R and sham surgery group. Moreover, I/R surgery significantly worsened the infiltration of macrophages into heart tissue compared with sham surgery.
Conclusions
Taken together, these findings suggested that myocardial I/R altered the gut microbiota profile, which may promote inflammatory cell infiltration in the injured heart.
Keywords
Ischemia-reperfusion injury, Gut microbiota, Inflammation
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Liang Qiu
Jiangxi University of Traditional Chinese Medicine
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5850-1455
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Jan, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The gut microbiota plays important roles in the development of cardiovascular diseases. However, whether myocardial ischemia–reperfusion (I/R) alters gut microbiota composition and its significance remains unknown. In this work, Sprague–Dawley rats were subjected to left anterior descending coronary artery ligation and reperfusion. The fecal DNA isolated before myocardial ischemia and after reperfusion were analyzed for microbiota changes by high-throughput 16S rDNA sequencing.
Results
Results showed that compared with the pre-I/R and pre-sham surgery group, I/R and sham surgery significantly increased the relative abundance of the phyla Proteobacteria , and decreased the relative abundance of the phylum Firmicutes . The I/R surgical procedure increased the proportions of phylum Spirochaetes and genus Enterococcus compared to pre-I/R and sham surgery group. Moreover, I/R surgery significantly worsened the infiltration of macrophages into heart tissue compared with sham surgery.
Conclusions
Taken together, these findings suggested that myocardial I/R altered the gut microbiota profile, which may promote inflammatory cell infiltration in the injured heart.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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