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Research article
Mediation of The Salicylic Acid Pathway by ROS1 in Response to Abiotic Stresses
Liping Yang
Institute of Life Sciences
Corresponding Author
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Background: DNA methylation plays an important role in the growth and development of plants in response to various abiotic stresses. Salicylic acid (SA) is an important signaling molecule that is synthesized by plants and induces the expression of defense genes.
Results: In this paper, we investigated the molecular mechanisms by which an upstream regulator (ACD6) in the SA pathway, an ABA pathway-related gene (ACO3), and a stress resistance gene (GSTF14) were induced by various abiotic stresses. The results demonstrated that abiotic stresses, including drought, cold, and salt stresses, induced the demethylation of the repeats in the promoters of ACD6, ACO3, and GSTF14 and transcriptionally activated their expression. Furthermore, our results revealed that ROS1-mediated DNA demethylation plays an important role in the process of transcriptional activation of ACD6 and GSTF14 when Arabidopsis plants were under cold stress.
Conclusions: Our results confirmed that ROS1 plays an important role in the process of defense genes in the SA pathway and stress resistance gene GSTF14 in response to abiotic stresses.
Keywords
DNA methylation, salicylic acid, ROS1-mediated DNA demethylation, abiotic stresses
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CURRENT STATUS: Under Review
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Posted 16 Dec, 2020
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Review #1 received
Received 15 Dec, 2020
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Received 15 Dec, 2020
Reviewer #2 agreed
On 14 Dec, 2020
Reviewer #1 agreed
On 13 Dec, 2020
Editor assigned
On 10 Dec, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Mediation of The Salicylic Acid Pathway by ROS1 in Response to Abiotic Stresses
Liping Yang
Institute of Life Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Dec, 2020
No community comments so far
Review #1 received
Received 15 Dec, 2020
Review #2 received
Received 15 Dec, 2020
Reviewer #2 agreed
On 14 Dec, 2020
Reviewer #1 agreed
On 13 Dec, 2020
Editor assigned
On 10 Dec, 2020
Reviewers invited
Invitations sent on 10 Dec, 2020
Submission checks complete
On 10 Dec, 2020
Editor invited
On 10 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: DNA methylation plays an important role in the growth and development of plants in response to various abiotic stresses. Salicylic acid (SA) is an important signaling molecule that is synthesized by plants and induces the expression of defense genes.
Results: In this paper, we investigated the molecular mechanisms by which an upstream regulator (ACD6) in the SA pathway, an ABA pathway-related gene (ACO3), and a stress resistance gene (GSTF14) were induced by various abiotic stresses. The results demonstrated that abiotic stresses, including drought, cold, and salt stresses, induced the demethylation of the repeats in the promoters of ACD6, ACO3, and GSTF14 and transcriptionally activated their expression. Furthermore, our results revealed that ROS1-mediated DNA demethylation plays an important role in the process of transcriptional activation of ACD6 and GSTF14 when Arabidopsis plants were under cold stress.
Conclusions: Our results confirmed that ROS1 plays an important role in the process of defense genes in the SA pathway and stress resistance gene GSTF14 in response to abiotic stresses.
Figure 1
Figure 2
Figure 3
Figure 4