Gut and oral bacterial diversity of the lizard Diploderma splendidum investigated using metagenomic analysis
Gut and oral microbial communities are complex and play a key role in their co-evolution with their hosts. However, little is understood about the bacterial community in lizards. In this study, we first investigated the gut and oral bacterial community in Diploderma splendidum from Sichuan Province, China. Metagenomic analysis of feces and oral cavity samples showed distinct differences between Diploderma splendidum and Liolaemus parvus, and L. ruibali and Phymaturus williamsi species.
Bacteridetes, Firmicutes, Proteobacteria and Fusobacteria were the most abundant phyla in fecal samples. However, the composition of the gut bacterial community of insectivorous lizards (Diploderma splendidum) exhibited unique abundance of phyla Proteobacteria and Chlamydiae when compared with L. parvus, L. ruibali and P. williamsi. Furthermore, Proteobacteria were abundant in oral cavity samples, followed by Actinobacteria, Chlamydiae and Firmicutes. Most striking was that the phylum Chlamydiae was most common in the oral cavity of Diploderma splendidum, when compared with a carnivorous lizard (Varanus komodoensis). In addition, more than 26 bacterial species were detected in the gut and/or oral cavity that were identified as potential human pathogens.
In this study, metagenomic analysis was carried out to reveal the gut and oral microbiomes, which brought new insight into the complex bacterial community and ecology in Diploderma splendidum.
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Posted 17 Dec, 2020
On 31 Jan, 2021
Received 24 Jan, 2021
Received 24 Jan, 2021
On 06 Jan, 2021
On 02 Jan, 2021
On 21 Dec, 2020
Received 21 Dec, 2020
On 16 Dec, 2020
Invitations sent on 16 Dec, 2020
On 14 Dec, 2020
On 13 Dec, 2020
On 16 Nov, 2020
Gut and oral bacterial diversity of the lizard Diploderma splendidum investigated using metagenomic analysis
Posted 17 Dec, 2020
On 31 Jan, 2021
Received 24 Jan, 2021
Received 24 Jan, 2021
On 06 Jan, 2021
On 02 Jan, 2021
On 21 Dec, 2020
Received 21 Dec, 2020
On 16 Dec, 2020
Invitations sent on 16 Dec, 2020
On 14 Dec, 2020
On 13 Dec, 2020
On 16 Nov, 2020
Gut and oral microbial communities are complex and play a key role in their co-evolution with their hosts. However, little is understood about the bacterial community in lizards. In this study, we first investigated the gut and oral bacterial community in Diploderma splendidum from Sichuan Province, China. Metagenomic analysis of feces and oral cavity samples showed distinct differences between Diploderma splendidum and Liolaemus parvus, and L. ruibali and Phymaturus williamsi species.
Bacteridetes, Firmicutes, Proteobacteria and Fusobacteria were the most abundant phyla in fecal samples. However, the composition of the gut bacterial community of insectivorous lizards (Diploderma splendidum) exhibited unique abundance of phyla Proteobacteria and Chlamydiae when compared with L. parvus, L. ruibali and P. williamsi. Furthermore, Proteobacteria were abundant in oral cavity samples, followed by Actinobacteria, Chlamydiae and Firmicutes. Most striking was that the phylum Chlamydiae was most common in the oral cavity of Diploderma splendidum, when compared with a carnivorous lizard (Varanus komodoensis). In addition, more than 26 bacterial species were detected in the gut and/or oral cavity that were identified as potential human pathogens.
In this study, metagenomic analysis was carried out to reveal the gut and oral microbiomes, which brought new insight into the complex bacterial community and ecology in Diploderma splendidum.
Figure 1
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