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Research article
Gut and oral bacterial diversity of the lizard Diploderma splendidum investigated using metagenomic analysis
Peng Guo
Yibin University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Gut and oral microbial communities are complex and play a key role in their co-evolution with their hosts. However, little is understood about the bacterial community in lizards. In this study, we first investigated the gut and oral bacterial community in Diploderma splendidum from Sichuan Province, China. Metagenomic analysis of feces and oral cavity samples showed distinct differences between Diploderma splendidum and Liolaemus parvus, and L. ruibali and Phymaturus williamsi species.
Results
Bacteridetes, Firmicutes, Proteobacteria and Fusobacteria were the most abundant phyla in fecal samples. However, the composition of the gut bacterial community of insectivorous lizards (Diploderma splendidum) exhibited unique abundance of phyla Proteobacteria and Chlamydiae when compared with L. parvus, L. ruibali and P. williamsi. Furthermore, Proteobacteria were abundant in oral cavity samples, followed by Actinobacteria, Chlamydiae and Firmicutes. Most striking was that the phylum Chlamydiae was most common in the oral cavity of Diploderma splendidum, when compared with a carnivorous lizard (Varanus komodoensis). In addition, more than 26 bacterial species were detected in the gut and/or oral cavity that were identified as potential human pathogens.
Conclusions
In this study, metagenomic analysis was carried out to reveal the gut and oral microbiomes, which brought new insight into the complex bacterial community and ecology in Diploderma splendidum.
Keywords
Diploderma splendidum, microbiota, metagenomic analysis, diversity
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CURRENT STATUS: Under Review
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Posted 17 Dec, 2020
No community comments so far
Editorial decision: Reject after review
On 31 Jan, 2021
Review #2 received
Received 24 Jan, 2021
Review #3 received
Received 24 Jan, 2021
Reviewer #3 agreed
On 06 Jan, 2021
Reviewer #2 agreed
On 02 Jan, 2021
Reviewer #1 agreed
On 21 Dec, 2020
Review #1 received
Received 21 Dec, 2020
Editor assigned
On 16 Dec, 2020
Reviewers invited
Invitations sent on 16 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 13 Dec, 2020
First submitted
On 16 Nov, 2020
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Subject Areas
Small Animal Medicine
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Gut and oral bacterial diversity of the lizard Diploderma splendidum investigated using metagenomic analysis
Peng Guo
Yibin University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 17 Dec, 2020
No community comments so far
Editorial decision: Reject after review
On 31 Jan, 2021
Review #2 received
Received 24 Jan, 2021
Review #3 received
Received 24 Jan, 2021
Reviewer #3 agreed
On 06 Jan, 2021
Reviewer #2 agreed
On 02 Jan, 2021
Reviewer #1 agreed
On 21 Dec, 2020
Review #1 received
Received 21 Dec, 2020
Editor assigned
On 16 Dec, 2020
Reviewers invited
Invitations sent on 16 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 13 Dec, 2020
First submitted
On 16 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Small Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Gut and oral microbial communities are complex and play a key role in their co-evolution with their hosts. However, little is understood about the bacterial community in lizards. In this study, we first investigated the gut and oral bacterial community in Diploderma splendidum from Sichuan Province, China. Metagenomic analysis of feces and oral cavity samples showed distinct differences between Diploderma splendidum and Liolaemus parvus, and L. ruibali and Phymaturus williamsi species.
Results
Bacteridetes, Firmicutes, Proteobacteria and Fusobacteria were the most abundant phyla in fecal samples. However, the composition of the gut bacterial community of insectivorous lizards (Diploderma splendidum) exhibited unique abundance of phyla Proteobacteria and Chlamydiae when compared with L. parvus, L. ruibali and P. williamsi. Furthermore, Proteobacteria were abundant in oral cavity samples, followed by Actinobacteria, Chlamydiae and Firmicutes. Most striking was that the phylum Chlamydiae was most common in the oral cavity of Diploderma splendidum, when compared with a carnivorous lizard (Varanus komodoensis). In addition, more than 26 bacterial species were detected in the gut and/or oral cavity that were identified as potential human pathogens.
Conclusions
In this study, metagenomic analysis was carried out to reveal the gut and oral microbiomes, which brought new insight into the complex bacterial community and ecology in Diploderma splendidum.
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