Alternative Splicing of RNF180 Genes in Different Species Based on Comparative Genomics Analysis
Alternative splicing (AS) influences gene regulation, cell differentiation, and tissue development and is involved in many human diseases. The ring finger protein 180 (RNF180), a tumor suppressor gene, has four transcripts and seven predicted transcripts. However, the role of alternatively spliced products in the function and regulation of the RNF180 gene remain unknown. We used a comparative genomics approach to investigate RNF180 AS in different species. Conserved coding sequences, alternative splicing expression profiles and intron sequences were compared, and evolutionary selection pressure analyses of exons were performed. We found that the RNF180 zinc finger structure, which was related to major ubiquitination functions, was highly conserved and exon 5 was absent in many species. Comparisons with the corresponding intron revealed that exon 5 possessed high similarity. In exon pressure selection analysis, exon 6 was in the purifying selection, which corresponded to the zinc finger domain, while exons 7 and 8 faced positive selection evolution. Finally, there were multiple alternatively spliced forms of RNF180 in four transcripts. These results suggested that complex alternative splicing of the RNF180 gene occurred in multiple species. Partial splicing variants had evolutionarily conserved regions and functional region deletions.
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Posted 21 Dec, 2020
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Alternative Splicing of RNF180 Genes in Different Species Based on Comparative Genomics Analysis
Posted 21 Dec, 2020
On 22 Jan, 2021
On 18 Jan, 2021
On 18 Jan, 2021
On 18 Jan, 2021
On 18 Jan, 2021
On 18 Jan, 2021
Invitations sent on 18 Jan, 2021
On 12 Jan, 2021
On 17 Dec, 2020
On 17 Dec, 2020
On 16 Dec, 2020
Alternative splicing (AS) influences gene regulation, cell differentiation, and tissue development and is involved in many human diseases. The ring finger protein 180 (RNF180), a tumor suppressor gene, has four transcripts and seven predicted transcripts. However, the role of alternatively spliced products in the function and regulation of the RNF180 gene remain unknown. We used a comparative genomics approach to investigate RNF180 AS in different species. Conserved coding sequences, alternative splicing expression profiles and intron sequences were compared, and evolutionary selection pressure analyses of exons were performed. We found that the RNF180 zinc finger structure, which was related to major ubiquitination functions, was highly conserved and exon 5 was absent in many species. Comparisons with the corresponding intron revealed that exon 5 possessed high similarity. In exon pressure selection analysis, exon 6 was in the purifying selection, which corresponded to the zinc finger domain, while exons 7 and 8 faced positive selection evolution. Finally, there were multiple alternatively spliced forms of RNF180 in four transcripts. These results suggested that complex alternative splicing of the RNF180 gene occurred in multiple species. Partial splicing variants had evolutionarily conserved regions and functional region deletions.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5