Background: Interstitial cystitis (IC) is a chronic inflammatory disease of the bladder, while both Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3 are the molecule related inflammatory diseases.
Objective: This study sought to investigate the potential feasibility of diagnosis of interstitial cystitis by measurement of the urinary level of Chemokine (C-X-C motif) ligand 7 (CXCL-7) and Galectin-3.
Methods: Both interstitial cystitis patients with or without Hunner’s ulcers were recruited in the study, while healthy subjects were involved as controls. Then we collected urine samples from all the involved subjects, and the CXCL-7 level and Galectin-3 level in the supernatants of urine were measured by ELISA. Urinary creatinine concentrations (mg Cr) of involved subjects were measured to normalize the level of CXCL-7 and Galectin-3. Also the clinical data and demographic characteristics of the recruited subjects were collected. The Receiver-operating curve (ROC) analysis was performed to explore the sensitivity and specialty of both CXCL-7 and Galectin-3 to identify interstitial cystitis. And the associations between urinary levels (CXCL-7 or Galectin-3) and the symptom severity (O'Leary and Sant Symptom, OSSI index) were analyzed with linear regression.
Results: The urinary levels of both CXCL-7 and Galectin-3 were significantly increased in interstitial cystitis compared to the healthy controls. The urinary CXCL-7 showed the sensitivity of 59.5% and specificity of 94.6%, at the cut-off value 21.30 pg/mg Cr to discriminate interstitial cystitis from controls, while the urinary Galectin-3 level showed the sensitivity of 62.2% and specificity of 91.9%, at the cut-off value 11.32 pg/mg Cr to discriminate interstitial cystitis from controls. Urinary Galectin-3 level was higher in interstitial cystitis patients with Hunner’s ulcers compared to patients without Hunner’s ulcers. The sensitivity and specificity were 45.5% and 93.3%, respectively for urinary CXCL-7 to discriminate from interstitial cystitis patients with Hunner’s ulcers to patients without Hunner’s ulcers, at the cut-off value of 35.97 pg/mg Cr. In addition, the urinary Galectin-3 level was positive correlated with the symptom severity.
Conclusion: CXCL-7 and Galectin-3 were elevated in the interstitial cystitis patients, both of which could be supplementary biomarker for diagnosis of interstitial cystitis. Moreover, urinary CXCL-7 might serve as the biomarker to decimate interstitial cystitis with Hunner ‘s ulcers from the without Hunner’s ulcers.