The protocol of the current study was approved by the ethics committee of Cerrahpaşa Medical Faculty (No: 8304 5809- 604.01.02). This was a retrospective study that conducted in the İstanbul University-Cerrahpaşa, Cerrahpaşa Medical Faculty, Department of Medical Oncology, from January 2019 to February 2020, 80 patients (mean age: 58,09 years; 30% female, 50% male) with colon cancer were enrolled in the study. There was no distant organ metastasis in all patients.
The patient’s age, sex, medical history, in preoperative blood routine examination as PLTs and fibrinogen levels were obtained from the patient’s medical record. All patients were of Turkish descent. Postoperative histopathological reports regarding tumor, lymph node involvement (negative or positive), and venous invasion (negative or positive) were obtained. The patient potentially curative surgery was defined. None of the patients received preoperative chemotherapy, radiotherapy or immunotherapy.
Patients with incomplete follow-up data or active concurrent infection were excluded. Patients were confirmed to have no distant organ metastasis. A hematologic disease or other infectious which could influence fibrinogen levels; liver disease, kidney disease, myocardial infarction, hypertension, use of procoagulant or anticoagulant drugs, the enrollment and a history of blood transfusion were also excluded from the study. The colon cancer patients were evaluated findings of physical tests, routine laboratory tests, diagnostic imaging (computed tomography, positron emission tomography), puncture biopsies and postoperative pathology reports.
Blood samples were obtained before surgery, blood samples were taken into standardized tubes containing ethylenediaminetetraacetic acid (EDTA) for complete blood count (CBC) parameters [platelet (PLT) (× 103/µL)] were obtained with automatic hematology analyzer (Beckman Coulter Brea CA, USA) plasma fibrinogen was measured by using Clauss method using Fibrintimer II coagulometer and Multifibren U kit (Siemens Health Care Diagnostics, Germany) İn preoperative blood routine examination and PLTs were obtained from the patient’s medical record. The manufacturer recommended normal ranges for plasma fibrinogen concentrations and PLT counts were 180–350 mg/dL and 15.6–34.8 × 104/µL, respectively.
Statistical analyses were performed using SPSS 20.0 (SPSS Inc; Chicago, IL, USA). All data were first checked for normally. Normally distributed continious variables are presented as mean ± standard deviasyon (SD) and were analyzed by one- way analysis of variance (ANOVA) followed by Tukey’s multiple comparison tests Pearson and Spearman’s correlations were used for numerical and nominal data respectively.