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Research
Hong-Fei Xia
Reproductive and Genetic Center of National Research Institute for Family Planning
Corresponding Author
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Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study ， the model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects.
Methods : The model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw. Bone collagen particles combined with hUC-MSCs were implanted in the defect area. Blood biochemical analysis was performed after 3 months. Skull tissues were harvested for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery.
Results: The bone collagen particles and HUC-MSCs have good biological safety. In addition, both can promote the regeneration of incisor. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration.
Conclusions: The method of HUC-MSCs combined with bone collagen particle material to fill a bone defect site is simple, rapid and suitable for the treatment of alveolar cleft bone defects.
Keywords
alveolar cleft, Bone collagen particles, HUC-MSCs (human umbilical cord mesenchymal stem cells), micro-focus computerized tomography (micro-CT)
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View the published article at BioMedical Engineering OnLine.
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On 18 Apr, 2020
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On 16 Apr, 2020
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Received 17 Mar, 2020
Review #1 received
Received 17 Mar, 2020
Editorial decision: Major revision
On 17 Mar, 2020
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On 14 Mar, 2020
Review #3 received
Received 14 Mar, 2020
Editor assigned
On 13 Mar, 2020
Reviewers invited
Invitations sent on 13 Mar, 2020
Reviewer #1 agreed
On 13 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Submission checks complete
On 12 Mar, 2020
Editor invited
On 12 Mar, 2020
No comments provided
Editorial decision: Revise before peer review
On 13 Feb, 2020
Editor assigned
On 04 Feb, 2020
First submitted
On 03 Feb, 2020
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On 03 Feb, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BioMedical Engineering OnLine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Hong-Fei Xia
Reproductive and Genetic Center of National Research Institute for Family Planning
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BioMedical Engineering OnLine.
Version 3
Posted 21 Apr, 2020
No community comments so far
Editorial decision: Major revision
On 18 Apr, 2020
Editor assigned
On 16 Apr, 2020
Submission checks complete
On 15 Apr, 2020
Editor invited
On 15 Apr, 2020
No comments provided
Review #2 received
Received 17 Mar, 2020
Review #1 received
Received 17 Mar, 2020
Editorial decision: Major revision
On 17 Mar, 2020
Reviewer #3 agreed
On 14 Mar, 2020
Review #3 received
Received 14 Mar, 2020
Editor assigned
On 13 Mar, 2020
Reviewers invited
Invitations sent on 13 Mar, 2020
Reviewer #1 agreed
On 13 Mar, 2020
Reviewer #2 agreed
On 13 Mar, 2020
Submission checks complete
On 12 Mar, 2020
Editor invited
On 12 Mar, 2020
No comments provided
Editorial decision: Revise before peer review
On 13 Feb, 2020
Editor assigned
On 04 Feb, 2020
First submitted
On 03 Feb, 2020
Submission checks complete
On 03 Feb, 2020
Editor invited
On 03 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BioMedical Engineering OnLine.
Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study ， the model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects.
Methods : The model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw. Bone collagen particles combined with hUC-MSCs were implanted in the defect area. Blood biochemical analysis was performed after 3 months. Skull tissues were harvested for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery.
Results: The bone collagen particles and HUC-MSCs have good biological safety. In addition, both can promote the regeneration of incisor. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration.
Conclusions: The method of HUC-MSCs combined with bone collagen particle material to fill a bone defect site is simple, rapid and suitable for the treatment of alveolar cleft bone defects.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
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