Acknowledgements
The authors acknowledge Dr. Benham Lockhart and the University of Minnesota Imaging Center for assistance with virus purification and particle visualization at the transmission electron microscopy facility.
Compliance with ethical standards
Ethics approval
This article does not contain any studies with human participants or animals performed by any of the authors. Plant samples were collected under Genetic Resource Access Permit # MAE–DNB–CM–2018–0098 granted by the Department of Biodiversity of the Ecuadorean Ministry of the Environment.
Funding
The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.
Conflict of interest declaration
The authors declare no conflict of interest.
Author contributions
Study conception and design: Diego F. Quito-Avila. Material preparation, data collection and analysis were performed by Maria G. Cañada-Bautista, Edison G. Reyes-Proaño, Juan F. Cornejo-Franco, Robert A. Alvarez-Quinto and Dimitre Mollov. The first draft of the manuscript was written by Maria G. Cañada-Bautista and Diego F. Quito-Avila; all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
Data Availability
The genomic sequences of the two virus isolates reported here have been deposited in the GenBank under accession numbers: OM263475 and OM263476.
Online Resource 1 Transmission electron micrograph of partially purified extracts from symptomatic leaves of Thevetia ahouai. A representative single flexuous filamentous virus-like particle is portrayed. Scale bar equals 100 nm. Magnification 60,000 X.
