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Research Article
Jinling Li
College of Chemistry and Chemical Engineering, Shaanxi Oil and Gas Pollution Control and Reservoir Protection Key Laboratory, Xi’an Shiyou University, Xi’an, 710065, China.
Corresponding Author
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The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioremediation + biostimulation was applied to improve the bioremediation effect of the soil with high oil content, that modified by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efficiency with respect to the total petroleum hydrocarbon (TPH) content reached 76.9% ± 2.2%; the soil bacterial content reached 4.9 × 107 CFU/g soil and the results were better than those in the natural attenuation M1group of experimental soils. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments.
Keywords
Clay with high oil content, Modified clay, Biostimulation and bioaugmentation, Soil ecological evaluation
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CURRENT STATUS: Under Review
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Posted 30 Dec, 2020
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Editorial decision: Major revision
On 19 Jan, 2021
Reviews received
Received 07 Jan, 2021
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On 25 Dec, 2020
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Jinling Li
College of Chemistry and Chemical Engineering, Shaanxi Oil and Gas Pollution Control and Reservoir Protection Key Laboratory, Xi’an Shiyou University, Xi’an, 710065, China.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 30 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 19 Jan, 2021
Reviews received
Received 07 Jan, 2021
Reviewers agreed
On 25 Dec, 2020
Reviewers invited
Invitations sent on 24 Dec, 2020
Editor assigned
On 24 Dec, 2020
Editor invited
On 24 Dec, 2020
Submission checks complete
On 24 Dec, 2020
First submitted
On 22 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The clay with high oil content form soil lumps, which is hard for microbes to repair. In this paper, the bioremediation + biostimulation was applied to improve the bioremediation effect of the soil with high oil content, that modified by local cow dung and sandy soil, the ecological toxicity of the soil after restoration was further analyzed. After 53 days of bioremediation, the degradation efficiency with respect to the total petroleum hydrocarbon (TPH) content reached 76.9% ± 2.2%; the soil bacterial content reached 4.9 × 107 CFU/g soil and the results were better than those in the natural attenuation M1group of experimental soils. The relative abundances of petroleum-degrading bacteria added to M5 remained high (Achromobacter 9.44%, Pseudomonas 31.06%, and Acinetobacter 14.11%), and the proportions of some other indigenous bacteria (Alcanivorax and Paenibacillus) also increased. The toxicity of the bioremediated soil was reduced by seed germination and earthworm survival experiments.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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