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Study protocol
PCR-based Detection of Diarrheagenic Pathogens in Improved Water Sources: a Scoping Review Protocol of the Evidence in Low- and Middle-income Countries
Shibabaw Tadesse Gemeda
Addis Ababa University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8253-4249
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Occurrence of diverse human enteric bacterial, viral and protozoal pathogens in drinking water samples because of fecal contamination is of increasing public health concern, particularly in developing counties. Application of Polymerase Chain Reaction (PCR) based methods in detecting microbial quality of water provides more accurate, sensitive and rapid outcomes over conventional methods of microbial identification and quantification. Therefore, exploring water quality outcomes generated through PCR based methods is important to better understand the status and monitor progress towards internationally set goals for low- and middle-income countries. The aim of this scoping review is to map the existing evidence on the magnitude and characteristics of diarrheagenic pathogens as detected by PCR-based methods in improved water sources within the context of low- and middle-income countries.
Methods: This scoping review will be undertaken in line with the JBI methodology for scoping reviews. We will consider studies from 2003 onwards that included PCR-based microbial water quality assessment of improved drinking water sources in low- and middle-income countries. The searches will be undertaken in PubMed/Medline, Scopus, Web of Science, JBI, Cochrane Library, and Google Scholar. A gray literature search will be conducted in Google and ProQuest.
Discussion: This review will systematically discover and integrate the evidence available on the detection of diarrheagenic pathogens through the application of PCR based methods for water quality determination. In this review, information about the applied PCR method, detected diarrheagenic pathogens, water sample size, effectiveness in terms of time and cost, will be gathered and summarized. Diagrammatic and/or tabular presentation supported with a narrative synthesis will be used. The review will be reported according to a reporting guideline for scoping reviews: PRISMA extension for scoping reviews (PRISMA ScR).
Keywords
Diarrheagenic pathogens, improved water, Polymerase chain reaction, Low- and middle-income countries
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CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
No community comments so far
Review #1 received
Received 16 Jan, 2021
Reviewer #2 agreed
On 28 Dec, 2020
Review #2 received
Received 28 Dec, 2020
Reviewer #1 agreed
On 27 Dec, 2020
Reviewers invited
Invitations sent on 26 Dec, 2020
First submitted
On 20 Dec, 2020
Editor assigned
On 20 Dec, 2020
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On 20 Dec, 2020
Editor invited
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This preprint is under consideration at Archives of Public Health. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Study protocol
PCR-based Detection of Diarrheagenic Pathogens in Improved Water Sources: a Scoping Review Protocol of the Evidence in Low- and Middle-income Countries
Shibabaw Tadesse Gemeda
Addis Ababa University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8253-4249
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
No community comments so far
Review #1 received
Received 16 Jan, 2021
Reviewer #2 agreed
On 28 Dec, 2020
Review #2 received
Received 28 Dec, 2020
Reviewer #1 agreed
On 27 Dec, 2020
Reviewers invited
Invitations sent on 26 Dec, 2020
First submitted
On 20 Dec, 2020
Editor assigned
On 20 Dec, 2020
Submission checks complete
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Occurrence of diverse human enteric bacterial, viral and protozoal pathogens in drinking water samples because of fecal contamination is of increasing public health concern, particularly in developing counties. Application of Polymerase Chain Reaction (PCR) based methods in detecting microbial quality of water provides more accurate, sensitive and rapid outcomes over conventional methods of microbial identification and quantification. Therefore, exploring water quality outcomes generated through PCR based methods is important to better understand the status and monitor progress towards internationally set goals for low- and middle-income countries. The aim of this scoping review is to map the existing evidence on the magnitude and characteristics of diarrheagenic pathogens as detected by PCR-based methods in improved water sources within the context of low- and middle-income countries.
Methods: This scoping review will be undertaken in line with the JBI methodology for scoping reviews. We will consider studies from 2003 onwards that included PCR-based microbial water quality assessment of improved drinking water sources in low- and middle-income countries. The searches will be undertaken in PubMed/Medline, Scopus, Web of Science, JBI, Cochrane Library, and Google Scholar. A gray literature search will be conducted in Google and ProQuest.
Discussion: This review will systematically discover and integrate the evidence available on the detection of diarrheagenic pathogens through the application of PCR based methods for water quality determination. In this review, information about the applied PCR method, detected diarrheagenic pathogens, water sample size, effectiveness in terms of time and cost, will be gathered and summarized. Diagrammatic and/or tabular presentation supported with a narrative synthesis will be used. The review will be reported according to a reporting guideline for scoping reviews: PRISMA extension for scoping reviews (PRISMA ScR).