Livestock is an important integral part to the sustainability of economy of this state. Antibiotic resistance is worldwide very common problem. The current study aimed to assess the knowledge, attitude, and practices pertaining to antibiotic usage among the field veterinarians.Feedback of 291 veterinary officers of Maharashtra state was taken regarding the use and resistance of different types of antibiotics in supplied format. It was revealed that ampicillin and amoxicillin in “Penicillin group”, oxytetracycline in “Tetracycline group”, cefotaxime and ceftriaxone in “Cephalosporine group”, enrofloxacine in “Quinolones group”, gentamycin in “Amynoglycosides group”,erythromycin in “Macrolides”group and Ampicillin &Cloxacillinin “Combination group” was used mostly due to their efficacy.The most important reason for using the antibiotics was found to be “effectiveness of antibiotic” (4.36 ± 0.08).It was indicated that in “bacterial infection” (4.62 ± 0.07) antibiotic was used mostly. Most important reason for antibiotic resistance was found to be “indiscriminate use of antibioticsby owners / paravets” (4.45 ± 0.08).The most important measure for prevention of antibiotic resistance was observed to be “ensuring proper dose and duration of treatment of antibiotics” (4.46 ± 0.08).