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Wen Chen
School of Public Health, Sun Yat-sen university
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5435-1664
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Background: Caloric restriction (CR) is known to improve health and extend life span in human beings. The effects of CR on adverse health outcomes in response to particulate matter (PM) exposure and the underlying mechanisms have yet to be defined.
Results: Male C57BL/6J mice were fed with CR diet or ad libitum (AL) and exposed to PM for 4 weeks in a real-ambient PM exposure system located at Shijiazhuang, China, with a daily mean concentration (95.77 μg/m³) of PM 2.5. Compared to AL-fed mice, CR-fed mice attenuated PM-induced pulmonary injury and systemic toxicity characterized by reduction in oxidative stress, DNA damage and inflammation. Analysis of RNA sequencing revealed that several pulmonary pathways involved in production of ROS, cytokine production, and inflammatory cell activation were inactivated, while those mediating antioxidant generation and DNA repair were activated in CR-fed mice upon PM exposure. In addition, transcriptome analysis of murine livers revealed that CR led to induction of xenobiotic metabolism and detoxification pathways, corroborated by increased levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and decreased cytotoxicity measured in an ex vivo assay.
Conclusion: These novel results demonstrate, for the first time, that CR in mice confers resistance against pulmonary injuries and systemic toxicity induced by PM exposure. CR led to activation of xenobiotic metabolism and enhanced detoxification of PM-bound chemicals. These findings provide evidence that dietary intervention may afford therapeutic means to reduce the health risk associated with PM exposure.
Keywords: Caloric restriction, particulate matter, pulmonary injury, systemic effects, RNA sequencing, xenobiotic metabolism.
Keywords
Caloric restriction, particulate matter, pulmonary injury, systemic effects, RNA sequencing, xenobiotic metabolism
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CURRENT STATUS: Published
View the published article at Particle and Fibre Toxicology.
No community comments so far
Review #2 received
Received 15 May, 2020
Review #1 received
Received 15 May, 2020
Reviewer #3 agreed
On 14 May, 2020
Editor assigned
On 13 May, 2020
Reviewers invited
Invitations sent on 13 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Reviewer #2 agreed
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
Version 1
Posted 10 Feb, 2020
No comments provided
Editorial decision: Major Revision
On 06 Apr, 2020
Review #3 received
Received 25 Mar, 2020
Reviewer #3 agreed
On 20 Mar, 2020
Review #2 received
Received 12 Mar, 2020
Review #1 received
Received 12 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Reviewer #2 agreed
On 10 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Editor assigned
On 06 Feb, 2020
First submitted
On 05 Feb, 2020
Submission checks complete
On 05 Feb, 2020
Editor invited
On 05 Feb, 2020
Metrics
Comments: 0
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Subject Areas
Toxicology
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A new preprint design is coming!
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See the published version of this preprint at Particle and Fibre Toxicology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Wen Chen
School of Public Health, Sun Yat-sen university
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5435-1664
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Particle and Fibre Toxicology.
No community comments so far
Review #2 received
Received 15 May, 2020
Review #1 received
Received 15 May, 2020
Reviewer #3 agreed
On 14 May, 2020
Editor assigned
On 13 May, 2020
Reviewers invited
Invitations sent on 13 May, 2020
Reviewer #1 agreed
On 13 May, 2020
Reviewer #2 agreed
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
Version 1
Posted 10 Feb, 2020
No comments provided
Editorial decision: Major Revision
On 06 Apr, 2020
Review #3 received
Received 25 Mar, 2020
Reviewer #3 agreed
On 20 Mar, 2020
Review #2 received
Received 12 Mar, 2020
Review #1 received
Received 12 Mar, 2020
Reviewer #1 agreed
On 10 Mar, 2020
Reviewer #2 agreed
On 10 Mar, 2020
Reviewers invited
Invitations sent on 09 Mar, 2020
Editor assigned
On 06 Feb, 2020
First submitted
On 05 Feb, 2020
Submission checks complete
On 05 Feb, 2020
Editor invited
On 05 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Toxicology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Particle and Fibre Toxicology.
Background: Caloric restriction (CR) is known to improve health and extend life span in human beings. The effects of CR on adverse health outcomes in response to particulate matter (PM) exposure and the underlying mechanisms have yet to be defined.
Results: Male C57BL/6J mice were fed with CR diet or ad libitum (AL) and exposed to PM for 4 weeks in a real-ambient PM exposure system located at Shijiazhuang, China, with a daily mean concentration (95.77 μg/m³) of PM 2.5. Compared to AL-fed mice, CR-fed mice attenuated PM-induced pulmonary injury and systemic toxicity characterized by reduction in oxidative stress, DNA damage and inflammation. Analysis of RNA sequencing revealed that several pulmonary pathways involved in production of ROS, cytokine production, and inflammatory cell activation were inactivated, while those mediating antioxidant generation and DNA repair were activated in CR-fed mice upon PM exposure. In addition, transcriptome analysis of murine livers revealed that CR led to induction of xenobiotic metabolism and detoxification pathways, corroborated by increased levels of urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and decreased cytotoxicity measured in an ex vivo assay.
Conclusion: These novel results demonstrate, for the first time, that CR in mice confers resistance against pulmonary injuries and systemic toxicity induced by PM exposure. CR led to activation of xenobiotic metabolism and enhanced detoxification of PM-bound chemicals. These findings provide evidence that dietary intervention may afford therapeutic means to reduce the health risk associated with PM exposure.
Keywords: Caloric restriction, particulate matter, pulmonary injury, systemic effects, RNA sequencing, xenobiotic metabolism.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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