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Research
Excessive Hormones Concentration in Serum May Increase the Risk of Polyspermy for Single Oocyte
Wenjun Wang
Sun Yat-Sen University 2nd Affiliated Hospital: Sun Yat-Sen Memorial Hospital
Corresponding Author
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Background: Fertilization of an oocyte by multiple sperms is known as polyspermy. Here, the purpose of this work was to investigate the possible correlation between hormones concentration in serum and the fertilization outcome of single oocyte in IVF cycles.
Methods: In this retrospective analysis, the baseline characteristics and serum hormones levels of female patients retrieving single oocyte in IVF cycles were analyzed. Female age, duration of infertility, body mass index (BMI), basal follicle stimulating hormone (FSH), basal luteinizing hormone (LH), basal estradiol (E2), the level of FSH, LH, E2 and progesterone (P) on the day of Gonadotropin (Gn), the level of FSH, LH, E2 and P on the day of human chorionic gonadotropin (HCG) were measured.
Results: According to the number of pronucleus in single oocyte on Day1, patients were assigned to IVF-2PN group (two pronucleus) and IVF-3PN group (three or more pronucleus). There were extremely higher levels of basal E2, LH and E2 on the day of Gn in IVF-3PN group compared with IVF-2PN group (P<0.01). The significantly higher level of LH on the day of HCG was present in IVF-3PN compared to IVF-2PN group (P<0.05). Forthermore, the optimal cutoff value of basal E2 (70.5 IU/L), LH (6.315 IU/L) and E2 (128.5 IU/L) on the day of Gn and LH (3.28 IU/L) on the day of HCG were determined by ROC curve analysis. The results showed that the percentage of IVF-2PN significantly decreased and the percentage of IVF-3PN considerably increased when the basal E2 concentration was higher than the cutoff value (P<0.05), the same tendency were present in the analysis of LH concentration, E2 concentration on the day of Gn and LH concentration on the day of HCG (P<0.01).
Conclusions: Our data indicated for the first time that the excessive concentration of basal E2, excessive concentrations of LH and E2 on the day of Gn and excessive concentration of LH on the day of HCG may significantly increase the risk of polyspermy for single oocyte in IVF cycles.
Keywords
IVF, Single oocyte, Hormone concentration, Fertilization outcome
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Research
Excessive Hormones Concentration in Serum May Increase the Risk of Polyspermy for Single Oocyte
Wenjun Wang
Sun Yat-Sen University 2nd Affiliated Hospital: Sun Yat-Sen Memorial Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Sexual & Reproductive Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Fertilization of an oocyte by multiple sperms is known as polyspermy. Here, the purpose of this work was to investigate the possible correlation between hormones concentration in serum and the fertilization outcome of single oocyte in IVF cycles.
Methods: In this retrospective analysis, the baseline characteristics and serum hormones levels of female patients retrieving single oocyte in IVF cycles were analyzed. Female age, duration of infertility, body mass index (BMI), basal follicle stimulating hormone (FSH), basal luteinizing hormone (LH), basal estradiol (E2), the level of FSH, LH, E2 and progesterone (P) on the day of Gonadotropin (Gn), the level of FSH, LH, E2 and P on the day of human chorionic gonadotropin (HCG) were measured.
Results: According to the number of pronucleus in single oocyte on Day1, patients were assigned to IVF-2PN group (two pronucleus) and IVF-3PN group (three or more pronucleus). There were extremely higher levels of basal E2, LH and E2 on the day of Gn in IVF-3PN group compared with IVF-2PN group (P<0.01). The significantly higher level of LH on the day of HCG was present in IVF-3PN compared to IVF-2PN group (P<0.05). Forthermore, the optimal cutoff value of basal E2 (70.5 IU/L), LH (6.315 IU/L) and E2 (128.5 IU/L) on the day of Gn and LH (3.28 IU/L) on the day of HCG were determined by ROC curve analysis. The results showed that the percentage of IVF-2PN significantly decreased and the percentage of IVF-3PN considerably increased when the basal E2 concentration was higher than the cutoff value (P<0.05), the same tendency were present in the analysis of LH concentration, E2 concentration on the day of Gn and LH concentration on the day of HCG (P<0.01).
Conclusions: Our data indicated for the first time that the excessive concentration of basal E2, excessive concentrations of LH and E2 on the day of Gn and excessive concentration of LH on the day of HCG may significantly increase the risk of polyspermy for single oocyte in IVF cycles.
Figure 1